Hi,
Do you have any CRISPR/Cas9 system tool such as in silico PCR? I designed a crispr vector, before order it, I want to check.
Thanks.
Hi, I have to re-create graphics that found at the articles. I did not understand Figure 3. I couldnot find raw data? How they were create the graphics? Can you explain?...
28 January 2024 7,893 3 View
Hi, I will do transfection with fugene but, I tried to enter three plasmids in cell. There is no selection part of plasmids, and I will try to plasmids enter to in cell one by one. How to do...
31 May 2023 1,940 3 View
Hi, I did western and you can see my beta actin bands. I put 10 uL primary antibody for my interesting protein, but my professors said 25 uL should be. I donot understand. Wouldn't I have seen a...
11 April 2023 8,649 2 View
Hello, I have band of my negative control. I am sure there is no contamination. I used new everything, sterile and change my gloves 5 times. I did negative control mix separately my samples. My...
02 January 2023 6,700 10 View
Hello, Do you have beta actin primer sequences for qPCR? I will study with 3T3 (mouse fibroblast), Raji (Human B cell), U937 (Human monocyte), HEK293 (Human embryonic kidney). May you suggest...
30 November 2022 9,372 0 View
Hello, Last week I asked my PCR results and I send Sanger seq. Well, there is one colony including mutation, I guess. How to cleaning my sample for Sanger? The Sanger result belongs to the second...
01 September 2022 7,808 4 View
Hi everyone, First of all, thank you for attention and answers. I did reverse transcriptase PCR for obtain cDNA from RNA. I used 3 different cell line RNA and my results looks fine....
19 April 2022 5,610 0 View
Hiii, I try to make mutations with QuikChange II XL Site-Directed Mutagenesis Kit. When I did first mutation, I got colonies and also mutation was succesfull. However, although I tried everything...
28 March 2022 1,919 3 View
Hi, I have a plasmid and designed site-directed mutagenesis primers, but my professor wants from me check plasmid with GFP after transformation? I donot know how to do that. Because our plasmid...
10 December 2021 6,245 4 View
Hi, I have 2 specific variants and i want to create mutatation with mutagenesis primers. How can I design these primers? I found QuickChange Primer Design Tool but I couldnot use. Do you have any...
23 October 2021 5,620 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,284 4 View