9 Questions 7 Answers 0 Followers
Questions related from Merve Gündoğdu
Hi, I will do transfection with fugene but, I tried to enter three plasmids in cell. There is no selection part of plasmids, and I will try to plasmids enter to in cell one by one. How to do...
01 June 2023 1,819 3 View
Hi, I did western and you can see my beta actin bands. I put 10 uL primary antibody for my interesting protein, but my professors said 25 uL should be. I donot understand. Wouldn't I have seen a...
12 April 2023 8,603 2 View
Hello, I have band of my negative control. I am sure there is no contamination. I used new everything, sterile and change my gloves 5 times. I did negative control mix separately my samples. My...
03 January 2023 6,652 10 View
Hello, Do you have beta actin primer sequences for qPCR? I will study with 3T3 (mouse fibroblast), Raji (Human B cell), U937 (Human monocyte), HEK293 (Human embryonic kidney). May you suggest...
01 December 2022 9,221 0 View
Hello, Last week I asked my PCR results and I send Sanger seq. Well, there is one colony including mutation, I guess. How to cleaning my sample for Sanger? The Sanger result belongs to the second...
02 September 2022 7,763 4 View
Hi everyone, First of all, thank you for attention and answers. I did reverse transcriptase PCR for obtain cDNA from RNA. I used 3 different cell line RNA and my results looks fine....
20 April 2022 5,568 0 View
Hiii, I try to make mutations with QuikChange II XL Site-Directed Mutagenesis Kit. When I did first mutation, I got colonies and also mutation was succesfull. However, although I tried everything...
29 March 2022 1,884 3 View
Hi, I have a plasmid and designed site-directed mutagenesis primers, but my professor wants from me check plasmid with GFP after transformation? I donot know how to do that. Because our plasmid...
11 December 2021 6,203 4 View
Hi, I have 2 specific variants and i want to create mutatation with mutagenesis primers. How can I design these primers? I found QuickChange Primer Design Tool but I couldnot use. Do you have any...
24 October 2021 5,581 3 View
