How can i perform the DPPH assay??. I Will use ethanol as solvent, and i will measure by spectrophotometer.
I want reference paper. Thank you
You can check this article maybe it can be helpful for you
Article Chemical composition, quality attributes, antioxidant activi...
first, prepare dpph solution (0.024g dpph in 100ml).
dilute your sample in different conc.
3ml dpph was taken and 200 μl of the sampl mixed and
incubate for 15min dark at RT.
The control was prepared exactly in the same way using 200 μl ethanol instead of a sample.
absorbance was measured at 517nm.
Asmaa Assiri
Assalam o alaikum wa rahmatullahi wa barkatuhu
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