How can i perform the DPPH assay??. I Will use ethanol as solvent, and i will measure by spectrophotometer.
I want reference paper. Thank you
You can check this article maybe it can be helpful for you
Article Chemical composition, quality attributes, antioxidant activi...
first, prepare dpph solution (0.024g dpph in 100ml).
dilute your sample in different conc.
3ml dpph was taken and 200 μl of the sampl mixed and
incubate for 15min dark at RT.
The control was prepared exactly in the same way using 200 μl ethanol instead of a sample.
absorbance was measured at 517nm.
Asmaa Assiri
Assalam o alaikum wa rahmatullahi wa barkatuhu
Here, a link of paper Article Phytochemicals, Antioxidant and Antiproliferative Studies of...
I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...
03 March 2021 7,540 4 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...
02 March 2021 1,404 6 View
I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.
01 March 2021 4,306 1 View
I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...
01 March 2021 9,525 4 View
28 February 2021 8,466 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...
28 February 2021 2,955 2 View
there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...
27 February 2021 2,609 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View