I have an excel file of differentially expressed genes that includes the logFC and the logCPM. These are a prior student's data so I did not create them and I do not need all of the genes, I only want to look into about 10 of the genes to understand if the absolute expression of the gene is biologically relevant and not just a massive fold change. I am new to Prism and just want to know the easiest way to go about this
If you have a spreadsheet with all the data, you can simply make a subset of desired data and plot. It can even be done in excel. Just choose the right columns for the right axis. You not necessarily need prism for that.
Hello Haidyn Bulen,
I agree and agree with Abhijeet Singh answer.
Moreover, if you are not familiar with the use of software such as "R" and that spreadsheets like Excell put you off, the recent versions of the statistical software GraphPad Prism allow to make representations (similar to MA plots or Volcano plot) and will allow you to visualize data in a 'classic' way in RNA-Seq analysis.
Please, see "How To Create A Volcano Plot In GraphPad Prism": https://youtu.be/oAB3jNspij0
In addition, HeatMap type representations are now available with GraphPad Prism.
Hoping that this comment will be useful. Feel free to discuss or share some informations about your RNA Seq data studies.
Ludovic Dumont, Ph.D.
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