We are planning to diagnose some infectious disease and genetic as well so can we used QuantStudio 5 PCR machine (Thermofisher).
thank you.
Dear Anil Kumar Sah,
I suggest you to see links and attached files on topic.
http://www.finddx.org/covid-19/pipeline/?avance=Commercialized&type=all&test_target=RNA&status=CE-IVD
https://www.thermofisher.com/dz/en/home/clinical/diagnostic-testing/instruments-automation/genetic-analysis-instruments/quantstudio-5-dx-real-time-pcr-system.html
https://www.onelambda.com/en/about-us/news/press-releases/PR-2017JUL31.html
https://www.bio-rad.com/en-dz/product/cfx96-dx-real-time-pcr-detection-systems-for-vitro-diagnostics-ivd?ID=NQH35P15
Article Evaluation of seven commercial RT‐PCR kits for COVID‐19 test...
Best regards
Dear,
May be, the following links, will help you.
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0014822_PresenceAbsence_UG.pdf
I believe it can be used as many have been using it for COVID diagnosis and FDA has approved this machine for diagnostics. Anil Kumar Sah
In metal oxides how oxygen affect the electrical and magnetic properties? Also, how it impacts the EMI shielding performance. Please make discussion.
05 June 2024 483 0 View
I want to make reconfigurable RF and microwave filter, I need to connect low capacitance (minmum-0.048pF) varactor diode (MA46H120 Series). Can we use this varactor diode (MA46H120 Series) at 6...
21 May 2024 7,504 2 View
We have elastic scattering excitation function data in tabular form and want to obtain partial wave scattering phase shift data for each partial wave say l=0, 1, 2....whats the process to do so...
19 April 2024 2,225 1 View
I have used the DLS machine (Zetasizer Software) to measure the size of micelle prepared. For the first 2 runs the quality report shows "Good", while the 3rd measurement shows as "Too...
17 April 2024 4,491 3 View
I have used ethanolic hcl reagent (95% ethanol and 1.5N Hcl in 85:15 ratio) for sample extraction. I got OD value at the range of 0.031 to 0.068. formula used : Total Anthocyanin mg/100g) = ( OD...
22 March 2024 4,417 1 View
I would like to see the Japanese patent with the number JP2206965, but I would like to see the entire text of it. I will be thankful to him if any of you are able to assist me.
16 February 2024 7,575 1 View
I am working on identification of copy number in a recombinant Chinese hamster cell line by southern hybridization. I am having issues with the transfer of DNA onto positively charged nylon...
08 February 2024 2,647 8 View
We did reaction of alpha-bromo-gamma-butyrolactone with aq. ammonia solution at RT to get alpha-amino-gamma-butyrolactone. Reaction completed on TLC. Water distilled completely but unable to...
07 February 2024 5,766 0 View
I tried different protein assay kits (BSA, fluorometric peptide), but it didn't work.
25 January 2024 3,341 0 View
For example, Data obtained from a rheometer is as follows 1. Static yield stress - 1400 Pa, 2. Dynamic yield stress- 640 Pa, 3. Plastic viscosity-68 Pa-Sec, How are these values exactly...
24 January 2024 6,120 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering
05 August 2024 1,660 0 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View