Molecular weight of Tris-Base - 121.1
Molecular weight of Tris-Hcl - 157.6
Can we use Tris-Hcl instead of Tris-base for preparation of TBE buffer and CTAB buffer?
I don't think so. Basically, Tris-HCl is a Tris-Base solution with HCl. If you think about the molecular weights of the two solution you will conclude that (Tris-Base = 121.1; H = 1,0079; Cl= 35,453 and so Tris-HCl is 121.1 + 1,0079 + 35,453 = 157.6). The point is that the salts composition is different in this two solutions so you could have problems related to the electolytes, expecially if you have to do an electrophoresis.
I don´t recommend it... I think better is to use Tris-base. Here is my "recipe" for 2.5 x concentrated TBE buffer for 0.5 L. 13.5 g Tris, 6.875 g boric acid, 5 ml 0.5 M EDTA (pH 8.0). As a running buffer I use 0.5 x concentrated TBE buffer (for RNA separation). To be honest, if you use Tris-HCl, it is not necessary to adjust pH by NaOH or HCl. So I´m not sure what do you exactly mean by Tris-HCl in this case. By mixing appropriate quantities of Tris-base and Tris-HCl, you can prepare stock buffers of various pHs.
I don't think so. Basically, Tris-HCl is a Tris-Base solution with HCl. If you think about the molecular weights of the two solution you will conclude that (Tris-Base = 121.1; H = 1,0079; Cl= 35,453 and so Tris-HCl is 121.1 + 1,0079 + 35,453 = 157.6). The point is that the salts composition is different in this two solutions so you could have problems related to the electolytes, expecially if you have to do an electrophoresis.
Adjusting the pH with NaOH/HCl tris will exercise its function tampon in a ideal range 7.06-9.06, so I do not think there is a problem . But the effect of the counterion (sodium / chloride) will depend on the system you use, if it is for a running buffer or separation of DNA, RNA or protein there will be no problem. If you are evaluating a system where you need to determine concentrations of chloride or sodium or something will have to consider the amounts present in your buffer.
You can use Tris base but pH should be adjust with 1N HCl. we got good results with Tris Base.
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