How can we estimate a genome size of any plant?

More Kamalesh Singh Mahar's questions See All

Can we isolate good quality cpDNA from dried tissue?

Genomic DNA, Chloroplast DNA, PCR

02 March 2017 6,275 2 View

Which method is commonly used for relationship of plant species?

There are many methods used in phylogeny viz. Maximum Likelihood Tree; Neighbor-Joining Tree; Minimum evolution Tree; UPGMA TREE & Maximum Parsimony Tree. I have simply two questions Whats a...

31 December 2016 2,487 9 View

How can we use the binary data generated by SPAR markers (RAPD, ISSR, DAMD etc.) for Biodiverse analysis software?

How can we use the binary data generated by SPAR markers (RAPD, ISSR, DAMD etc.) for Biodiverse analysis software? Biodiverse software (a tool for the spatial analysis of diversity) How do we make...

06 July 2015 1,581 6 View

Can we use Tris-Hcl instead of Tris-base for TBE buffer?

Molecular weight of Tris-Base - 121.1 Molecular weight of Tris-Hcl - 157.6 Can we use Tris-Hcl instead of Tris-base for preparation of TBE buffer and CTAB buffer?

10 November 2014 3,374 5 View

Whats a importance of mrbayes analysis in phylogenetics?

Phylogeny, molecular evolution

31 December 2013 8,605 1 View

Can we perform Bayesian phylogenetic analysis in MEGA 6?

Thanks in advance.

31 December 2013 2,068 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

How to calculate effect size of AMCE (Average Marginal Component Effect) in Randomized Conjoint Experiment?

I am following Hainmueller, Hopkins, and Yamamoto's (2014) paper for my randomized conjoint experimental data analysis. The link to the paper is provided below. I received a comment from the...

02 August 2024 4,406 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Bachir Achour

Dear Kamalesh,

I suggest you the following interesting papers.

http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.337.5114&rep=rep1&type=pdf

http://aob.oxfordjournals.org/content/95/1/99.full

http://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-13-219

https://www.researchgate.net/publication/271318442_Estimation_of_the_Genome_Size_of_Tea_Camellia_sinensis_Camellia_C_japonica_and_their_Interspecific_Hybrids_by_Flow_Cytometry

With my best regards

Prof. Bachir ACHOUR

Article Estimation of the Genome Size of Tea (Camellia sinensis), Ca...

Kamalesh Singh Mahar

Thanks Prof Bachir Achour for kind information's.

with best regards

Kamalesh S. Mahar

Arvind Singh

Please have a look at these links and PDF attachment.

https://www.researchgate.net/publication/51779349_Genome_Size_Determination_Using_Flow_Cytometry_of_Propidium_Iodide-Stained_Nuclei

https://www.researchgate.net/publication/225167844_Nuclear_DNA_Content_of_Some_Important_Plant_Species

Article Genome Size Determination Using Flow Cytometry of Propidium ...

Article Nuclear DNA Content of Some Important Plant Species

Kalyani Sunil Kulkarni

Flow cytometry

Whole genome sequencing and k-mer analysis

Thanks dear Arvind Singh and Kulkarni mam.

Can we estimate a genome size without flow cytometry and sequencing ?

Please refer the following paper

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC156059/

Thanks