Can we isolate good quality cpDNA from dried tissue?

More Kamalesh Singh Mahar's questions See All

Which method is commonly used for relationship of plant species?

There are many methods used in phylogeny viz. Maximum Likelihood Tree; Neighbor-Joining Tree; Minimum evolution Tree; UPGMA TREE & Maximum Parsimony Tree. I have simply two questions Whats a...

31 December 2016 2,487 9 View

How can we estimate a genome size of any plant?

Methods for estimation of Plant genome size

06 July 2016 9,484 6 View

How can we use the binary data generated by SPAR markers (RAPD, ISSR, DAMD etc.) for Biodiverse analysis software?

How can we use the binary data generated by SPAR markers (RAPD, ISSR, DAMD etc.) for Biodiverse analysis software? Biodiverse software (a tool for the spatial analysis of diversity) How do we make...

06 July 2015 1,581 6 View

Can we use Tris-Hcl instead of Tris-base for TBE buffer?

Molecular weight of Tris-Base - 121.1 Molecular weight of Tris-Hcl - 157.6 Can we use Tris-Hcl instead of Tris-base for preparation of TBE buffer and CTAB buffer?

10 November 2014 3,374 5 View

Whats a importance of mrbayes analysis in phylogenetics?

Phylogeny, molecular evolution

31 December 2013 8,605 1 View

Can we perform Bayesian phylogenetic analysis in MEGA 6?

Thanks in advance.

31 December 2013 2,068 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Arvind Singh

Please check the below given link.

https://www.researchgate.net/publication/225622256_DNA_isolation_from_dry_and_fresh_samples_of_polysaccharide-rich_plants

Article DNA isolation from dry and fresh samples of polysaccharide-rich plants

Surender Singh

Plz have a look on this link.

http://wgbis.ces.iisc.ernet.in/biodiversity/sahyadri_enews/newsletter/issue41/bibliography/DNA_Extraction_From_Processed_Wood.PDF