I am currently working on hydrogel for wound healing research. In this research, i am interested in scratch assay to assess/measure cell migration. In some paper that i have read, it said that in scratch assay, it preferably use serum free media or
My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...
28 July 2024 2,794 6 View
Dear All, I would like to ask about the relation between the surface energy and solidification of polymer. If we apply a chemical treatment such as NaOh solution or silane treatment on the...
17 July 2024 190 3 View
We have two types of polymers, high-density polyethene and polypropylene, with voids forming in the material. We observed the void at the cross-section of the material. The material is a recycled...
28 February 2024 9,588 2 View
Hello, currently, I am working on the chloroplast genome and experiencing difficulties in reading ambiguous code N in the intron. I have blasted it to NCBI, but there are no nucleotides aligned...
02 July 2023 1,703 0 View
Hello, I'm currently working on my final project about carbon stock estimation in seaweed. After taking the samples from the cultivation area, I have to dry them with oven to obtain the biomass....
04 December 2022 8,688 4 View
When replicating this paper Hybrid Evolutionary Algorithms for Graph Coloring,P. Galinier and J.K. Hao. Hybrid evolutionary algorithms for graph coloring. Journal of Combinatorial Optimization,...
19 January 2022 5,632 0 View
Hi, could anyone suggest which statistical analysis is used to analyze the data of pretest-posttest control group design with two dependent variables and one independent variable? your explanation...
09 September 2021 2,862 7 View
I am looking for some articles on microlearning and parental involvement. Thank you
12 July 2021 5,126 3 View
Hello everyone, Sometimes we feel confused when we want to focus on measuring or assessing someone's experience, because someone's experience has a very broad scope, both morally and spiritually....
05 January 2021 984 3 View
I hope to track my liposome nanoparticles both in vitro and in vivo. I also intend to quantify each excipient ratio from the corresponding labeling (if possible). Does anyone familiar or have some...
28 January 2020 7,051 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View