I hope to track my liposome nanoparticles both in vitro and in vivo. I also intend to quantify each excipient ratio from the corresponding labeling (if possible). Does anyone familiar or have some thought for this particular techniques? Any inputs would be greatly appreciated.
One option would be Deuterium Labeled Lipids (can be obtained from Avanti Polar lipids) like the one shown in the attached picture.
Dear researcher,
You can use radio-labeling and fluorescent-based tracer in order to track and follow you nano-carrier in in-vitro and in-vivo experiments. In what follows, I attached two useful manuscripts by which you can follow your work effectively:
1. Imaging, biodistribution and in vitro study of smart 99mTc-PAMAM G4 dendrimer as novel nano-complex
2. Targeting delivery of oxaliplatin with smart PEG-modified PAMAM G4 to colorectal cell line: In vitro studies
You can try something that is fluorescent, such as fluorescent liposomes or reversible fluorescent labels for your APIs
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