Hi,

you can perfectly use the far red dyes for facs. Those far red dye are like APC dyes.

Hi,

The Live/dead near IR will come out in APCCy7 channel. It works very well in flow cytometry.

Good luck,

Silvia

Dear Ramachandran,

The answer is yes.

The following describes a kit which is used for such cases:

LIVE/DEAD® Fixable Far Red Dead Cell Stain Kit, for 633 or 635 nm excitation

Catalog number: L10120

Specifications

Compatible Cells: Eukaryotic Cells

For Use With (Equipment): Flow Cytometer

Detection Method: Fluorescent

Format: Tube(s)

Form: Solid

Solubility: DMSO (Dimethylsulfoxide)

Label or Dye: LIVE/DEAD® Fixable Far Red Dead Cell Stain

Product Size: 200 assays

Number of Reactions: 200

Flow Cytometer Laser Lines: 633/635

Excitation⁄Emission (nm): 650⁄665

Shipping Condition: Room Temperature

Contents & storage

Contains 5 vials of LIVE/DEAD® fixable dead cell stain and 500 µL DMSO. Store at -20°C.

Optimal brightness

The LIVE/DEAD™ Fixable Far-Red Stain was selected based on its fluorescent properties to provide a bright signal when excited with a red laser. The far-red fluorescent reactive dye has an excitation maximum of ~633 nm making it ideal for use with the red or HeNe laser with an emission of ~655 nm. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments.

How it works

In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.

Colors available

LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.

Citations & references

CD8+ T cell responses to lytic EBV infection: late antigen specificities as subdominant components of the total response.

Abbott RJ, Quinn LL, Leese AM, Scholes HM, Pachnio A, Rickinson AB,

J Immunol (2013) 191:5398-5409

Product usage: Viability, Fixable, Far Red, Flow cytometry, CD8+, T cells

The oxysterol-CXCR2 axis plays a key role in the recruitment of tumor-promoting neutrophils.

Raccosta L, Fontana R, Maggioni D, Lanterna C, Villablanca EJ, Paniccia A, Musumeci A, Chiricozzi E, Trincavelli ML, Daniele S, Martini C, Gustafsson JA, Doglioni C, Feo SG, Leiva A, Ciampa MG, Mauri L, Sensi C, Prinetti A, Eberini I, Mora JR, Bordignon C, Steffensen KR, Sonnino S, Sozzani S, Traversari C, Russo V,

J Exp Med (2013) 210:1711-1728

Product usage: Viability, Fixable, Far Red, FACS

Hoping this will be helpful,

Rafik

Agree with karaman

Yes, you can use the far red invitrogen dye for FACS. Alll the invitrogen dyes to discriminate live and dead cells work very well. Good luck!

Hello

Agree with all researcher , you can use 

Good Luck 

https://www.pasteur.fr/ip/portal/action/WebdriveActionEvent/oid/01s-00002m-02o/fname/O-072942_FlowInstrGuide_final2%20(2).pdf

Yes, you absolutely can. 

Thank you all for your inputs

Hello,

Have anybody an experience of use LIVE/DEAD stain for live cells tracing? How about cell viability in the culture after staining?

Thanks!