Can we use dry ice in process of Dna extraction for breaking of cell membranes and freezing the specimen?

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Not able to give GAFF forcefield to my ligand?

I have been able to assign the CHARMM forcefield to my ligand i.e. acetyl coenzyme A but not the amber forcefield. I want to run amber forcefield and the parameterization of ligand is failing. I...

15 July 2024 7,323 1 View

While doing docking using Autodock1.5.7, I am getting a warning that there are non integral charges on my ligand. How to remove it?

I used Autodock for docking, due to the non integral charges on my ligand I am not able to dock with the protein. How to dock the file?

08 June 2024 5,593 2 View

Investigation on structural, hysteresis, Mössbauer properties and electrical parameters in X-type Ba2Co2ErxFe28-xO46 hexaferrites?

Conducted an extensive study on hexaferrites, substituting Erbium into X-type Ba2Co2ErxFe28-xO46 through a precise heat treatment process. XRD analysis highlighted phase variations, showcasing...

16 November 2023 2,147 2 View

How do I report a security concern?

Someone is trying to create a new account using my email. An activation request was forwarded to me to activate the email account severally earlier, and even today by 08:06am.

30 July 2023 9,835 0 View

How to determine the final weights in fuzzy AHP at subclass level?

I have a basic understanding of the fuzzy AHP process. But there are a few confusion during the calculation of weights when calculated at the sub-class level. Example: I have 5 layers...

12 June 2023 4,879 0 View

I'm facing problem in NO determination using Griess reagent of SRL ?

for standard i have prepared 10 mM stock and diluted (10 -1200 micromolar) added SRL Griess reagent placed 15 min incubation in dark in to various concentration for the standard graph preparation...

28 May 2023 586 0 View

How to perform Water column correction on a satellite image?

I am working on coral bleeching process where I need to make water column correction on landsat images. I am not able to understand how do to perform this tech and using what software ? can it be...

08 April 2023 2,975 0 View

Can anyone give information about CD4 and CD8 expression on EL-4 mouse T lymphoma cells. Any other marker to be used for checking stimulation?

I stained EL-4 mouse T cells with CD4 and CD8 in unstimulated and stimulated condition. but I am hardly seeing any CD8 expression. Do these cells not express CD8? if so are there any other markers...

19 March 2023 9,268 1 View

How we can apply six sigma in casting foudry industries ?

Impemtation of six sigma is very complex in casting process as we have to control so many parameters to reduce rejection. I want to discuss about this if some have any resarch or case study about it.

13 February 2023 315 4 View

How to quantify Phenol in selective oxidation of Benzene ??

In selective oxidation of Benzene, catalyzed by heterogeneous catalyst due to the volatile nature, quantification of unreacted Benzene is difficult which leads to wrong conclusion. Another thing...

18 May 2022 830 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View