Can we use dry ice in process of Dna extraction for breaking of cell membranes and freezing the specimen?

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General Researching Question: How to begin research?

I am in the 2nd year of my undergraduate and I am very much interested in doing research in biology, chemistry and physics! I saw research opportunities under my professors but they are too...

24 December 2020 1,475 6 View

To find out best pair of result using GA?

Hello everyone, I have results of 100 year for a particular station. Now I have two values the first is R^2 and second in SSE for every year. Now what I want that GA should find out best result...

01 January 2020 5,722 1 View

What are the techniques to find out optimal result?

Hello everyone, I am working with 10 years of data for which I have computed 10 results. But I need to show the best result out of 10 outcomes. Can anyone suggest me any methods to find out...

29 December 2019 7,710 2 View

I have doped ZnO nanoparticles using tungsten and antimony.1% doping..their atomic radius is different so doping would be possible or not?

i have analyzed samples using XRD, i got peaks with a slight peak shift for pure and doped material. no any addition peak was found. just XRD is enough to confirm the doping?

04 December 2019 2,030 3 View

Researches Going On Use Of Bio-technology In Field Of Construction Technology?

Like: Self Healing Concert, Smoke Absorbing Facades etc..,

15 August 2019 6,207 4 View

Is there a restriction in Amplicon size for ddPCR ?

Hello , We need to do ddPCR (Eva green chemistry) for a primer pair that yields a 429 bp amplicon. It is a single band on gel. I understand that there are reason for restriction amplicon size in...

22 April 2019 2,388 3 View

What are the boundary conditions required for the Fabry-Perot Cavity 3D model in COMSOL Multiphysics?

I want to model Fabry-Perot Cavity in COMSOL Multiphysics. 2D model is easy to make. I am willing to make a 3D model of Fabry-Perot Cavity.

17 March 2019 6,725 3 View

Is open innovation model works in the field of dairy?

open innovation framework is useful in the food and drink sector but i would like to know the relation between OI model w.r.t. Dairy packaging

07 December 2018 2,011 2 View

Need solution for solubility of BCAA?

I would highly appreciate if anyone can help me with how to make BCAA powder soluble. Is Sunflower Lecithine recommended? if yes, which form (powder or oil). Also how much per 100g of BCAA? If...

19 August 2018 9,861 2 View

I have a 10-kb PCR amplicon whose conc. drops drastically upon DpnI digestion. What could be the reason? How to improve yield?

We have a PCR amplicon which is around 10kb (plasmid backbone). We have to digest it with DpnI before proceeding with other steps. The gel extraction concentration is generally 1ug-1.5ug per 100ul...

23 April 2018 8,271 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View