Can anyone give information about CD4 and CD8 expression on EL-4 mouse T lymphoma cells. Any other marker to be used for checking stimulation?

20 March 2023 1 9K Report

I stained EL-4 mouse T cells with CD4 and CD8 in unstimulated and stimulated condition. but I am hardly seeing any CD8 expression. Do these cells not express CD8? if so are there any other markers I can test for EL-4 stimulation?

Amine Mellouk

In my knowledge, I think EL-4 cells do not express CD4 or CD8. I used CD90 (Thy.2) to be sure about the cell line origin (as murine T cell).

How do you stimulate your cells? if you stimulate the with PMA for example, they might produce more IL-2.

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