Can we use any reverse transcriptase enzyme with any type of buffer?

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Deyuan Dong

You'd better use the corresponding buffer in case of unknown circumstances.

Maxime Pichon

In case of commercial assays, I would recommend to use appropriate buffer to ensure optimal performances. An other RT buffer could also be used I think but performances might be lower ...

Nadia Jamil

Thank you Maxime Pichon and Deyuan Dong.