Nadia Jamil

9 Questions 15 Answers 0 Followers

Questions related from Nadia Jamil

Is OpenTech a predatory Journal?

I searched and it is not in https://beallslist.net/standalone-journals. I need guidance on it please.

02 July 2023 9,970 3 View

What is the proper method for cleaning of combs for agarose gel electrophoresis?

Can we wash it by boiling it in water if the visible stains of ethidium bromide are not washed after rinsing with water and then 70% ethanol?

07 August 2018 8,145 3 View

What does number written between sequence of nucleotide means?

If there is a number superscript on the sequence of a primer what does it means? e.g. T153AGCT etc

22 December 2017 4,897 1 View

Can anyone recommend me any tools or software's for Viral In silico PCR and predicting DNA bands?

I have seen following...

05 December 2017 8,151 3 View

My PCR band disappeared after optimization, what should I do?

I ran a PCR reaction and got my desired band at 800 bp with other non specific bands (reaction vol 25 ul) after optimization I got 800 bp band clear (reaction vol 25 ul). Then I went for bulk...

03 October 2017 3,975 7 View

Can anyone please suggest a good DNA polymerase and reverse transcriptase?

I need enzymes that can work with my in-house PCR buffer. Please suggest if there is any good DNA polymerase and reverse transcriptase, I also do not have a lot of funds so I would be really...

03 October 2017 1,483 3 View

Can we use any reverse transcriptase enzyme with any type of buffer?

when we use reverse transcriptase do we have to use specific buffer with that or any taq buffer can also work with that?

03 October 2017 5,786 3 View

Result of my BLAST says no significant similarity found, what does it mean?

Hi there I isolated bacteria from serum sample did the extraction of DNA for bacteria, followed by PCR. PCR band was of 100bp I sent it for sequencing. The results were approx 43 bp forward primer...

04 August 2017 3,301 9 View

What is the alternate for Beta Mercaptoethanol in DNA extraction method. If one does not have it?

I need to Isolate bacterial DNA of high quality but without using kit.

30 March 2017 6,529 6 View