In Western blots sometimes we get a band upper or lower than what expected. Can we trust these bands to verify the presence of a protein?
No. Even when the band is precisely at the expected molecular weight you won't be certain you have detected the protein you are interested in. Making a negative control lysate with siRNA knockdown should be done routinely.
Thanks dear Mark
Recently, I found a commercial kit (or reagent), by which in immunoprecipitation, it removes the precipitating antibody from the mixture and in WB you will not see the 50 and 100 kDa bands....
03 March 2019 3,640 3 View
02 March 2019 4,052 4 View
I made MCM-41 mesoporous silica with Gold inside but I cant disperse them in water..
18 November 2015 4,701 5 View
17 September 2013 2,296 2 View
These days with novel facilities we have better understandings of sciences. There are many documented findings that might not be really correct. For example: origin of leukemic cells in Chronic...
31 December 2012 1,994 3 View
The textbooks say we should always use the same concentration of isotype control as the test monoclonal Ab in flow cytometry assays to detect surface expression of a molecule. In my experiment,...
18 December 2012 4,526 21 View
11 December 2012 8,729 22 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View