Can we trust protein molecular weight markers to detect the presence of the right protein in Western blot?

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Remove antibody from coimmunoprecipitation mixture?

Recently, I found a commercial kit (or reagent), by which in immunoprecipitation, it removes the precipitating antibody from the mixture and in WB you will not see the 50 and 100 kDa bands....

03 March 2019 3,494 3 View

Remove antibody from coimmunoprecipitation mixture?

Recently, I found a commercial kit (or reagent), by which in immunoprecipitation, it removes the precipitating antibody from the mixture and in WB you will not see the 50 and 100 kDa bands....

02 March 2019 3,962 4 View

Can we trust protein molecular weight markers to detect the presence of the right protein in Western blot?

In Western blots sometimes we get a band upper or lower than what expected. Can we trust these bands to verify the presence of a protein?

17 September 2013 2,184 2 View

What do you think about questioning the well documented research findings?

These days with novel facilities we have better understandings of sciences. There are many documented findings that might not be really correct. For example: origin of leukemic cells in Chronic...

31 December 2012 1,909 3 View

What do you think about using only secoundary conjugated Ab in indirect flow cytometry when you do not have the proper unconjugated isotype control?

The textbooks say we should always use the same concentration of isotype control as the test monoclonal Ab in flow cytometry assays to detect surface expression of a molecule. In my experiment,...

18 December 2012 4,398 21 View

What do you think about using only secoundary conjugated Ab in indirect flow cytometry when you do not have the proper unconjugated isotype control?

The textbooks say we should always use the same concentration of isotype control as the test monoclonal Ab in flow cytometry assays to detect surface expression of a molecule. In my experiment,...

11 December 2012 8,641 22 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View