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Questions related from Mohammad Hojjat-Farsangi
Recently, I found a commercial kit (or reagent), by which in immunoprecipitation, it removes the precipitating antibody from the mixture and in WB you will not see the 50 and 100 kDa bands....
03 March 2019 4,018 4 View
In Western blots sometimes we get a band upper or lower than what expected. Can we trust these bands to verify the presence of a protein?
09 September 2013 8,395 2 View
These days with novel facilities we have better understandings of sciences. There are many documented findings that might not be really correct. For example: origin of leukemic cells in Chronic...
01 January 2013 1,957 3 View
The textbooks say we should always use the same concentration of isotype control as the test monoclonal Ab in flow cytometry assays to detect surface expression of a molecule. In my experiment,...
12 December 2012 8,689 22 View
