I need to perform competitive repopulation assays with CD45.1 FVB cells but I do not have congenic CD45.2 mice in FVB background. Can I cross CD45.1 FVB and CD45.2 Bl6, get F1 and then transplan CD45.1 FVB (P1) cells?
heavy use of Large Language Model will going to change the working style of organization?
23 June 2024 7,451 3 View
alarmins im interested in include: HMGB1 and S100 proteins amongst others Will other cell lysate proteins mask the signal from the alarmins im interested in?
30 May 2024 8,127 0 View
Can be publish any paper through research gate?
24 March 2024 10,037 3 View
Hello, I'm studying rainfall and temperature's impact on river basin water quality. Are there any indices that effectively capture both rainfall and temperature? I have only temperature and...
10 March 2024 7,224 3 View
Has anyone ever performed MASW using DAS acoustic sensing approach , as traditionally only geophone sensor are utilized. If so the answer is yes, how did they manage to acquire the datasets
29 February 2024 1,137 0 View
Resuscitation drugs become ineffective during severe metabolic acidosis ...why can't bicarbonate be a part of ACLS protocol
22 January 2024 5,099 3 View
I am looking to quantify metabolites released into cell media, especially alarmins/DAMPS and cytokines. The concentrations of these inflammatory proteins will be on the nanograms/ml or nanomolar...
03 January 2024 1,628 0 View
I have tried dissolving the same in DMSO and water at 1mg/ml, sonicated the stocks but there was precipitate formation and l could not get a clear solution.
10 October 2023 7,567 0 View
I have tried dissolving the same at 1mg/ml in both water and DMSO but there was precipitate formation. I even tried heating and sonication but there was no effect as there were precipitates.
10 October 2023 2,121 1 View
What are the factors affecting the measurement of electrolytic conductivity using DC and AC techniques? What are the challenges and precautions related to electrolytic conductivity measurement?...
26 June 2023 5,507 2 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View