I am doing acetone precipitation for partial purification of asparaginase from bacterial strains. Can I reuse acetone for the same purpose for the same strain. As it is used in bulk quantity for precipitation of protein 1 or 2 liter medium.
I'm working on IDS using machine learning on real traffic. basically, Lots of work has been done on datasets and I have found a very few works on testing on real traffic. So I want to know how can...
18 October 2020 2,485 3 View
Can someone please simulate and explain these protein-ligand interactions for each of the ligands: Please help me, it's urgent. Protein 5I08 : https://www.rcsb.org/structure/5I08 Ligands 1R42 :...
24 March 2020 4,144 4 View
Hi, I am analysing human peripheral blood by flow cytometry and I have to prepare FMO controls. If I have two or more samples from different persons then should I need to prepare FMO controls for...
27 October 2015 3,771 2 View
Researchers and Educationists,Don't you feel Its our duty to provide solutions and suggestions.
01 January 1970 4,250 3 View
Researchers kindly answer with statistics
01 January 1970 2,469 10 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...
19 February 2021 9,742 2 View
I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...
15 February 2021 3,746 4 View
Hello all, I am looking for ways to compare recharge of groundwater and determine if precipitation has more of an effect on groundwater levels before or after restoration? My data set contains a...
08 February 2021 7,887 5 View
I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...
04 February 2021 2,738 3 View
I have an alkaline solid waste which contains the following components: SiO2, Al2O3, and CaO according to the XRF analysis (as it gives the results as oxides). However, I know from the XRD that it...
28 January 2021 9,469 3 View
I've synthesized a polyurethane-Ag nanocomposite and the product is dissolved in a two-component system i.e. DMF and DMSO. I wish to know of any ways that I can use to get rid of the solvents and...
27 January 2021 1,847 3 View
Hello! I am precipitating some proteins (with either acetone or ammonium sulphate) and I need to resuspend the protein pellet in a buffer that properly keeps the proteins soluble AND which is...
27 January 2021 6,958 4 View
gold surface is already conjugated with the PEG-2000 linker but still when I tried to conjugate it with the drug, It gets precipitated
25 January 2021 2,602 1 View
I am doing HEC HMS calibration than i want to increase simulated value and volume of the computed so that my issue is how the precipitation and flow ratio make influential that result?
22 January 2021 152 2 View