I am doing acetone precipitation for partial purification of asparaginase from bacterial strains. Can I reuse acetone for the same purpose for the same strain. As it is used in bulk quantity for precipitation of protein 1 or 2 liter medium.
In the methodology of biosynthesis of silver nanoparticles
15 July 2023 3,768 4 View
Bi2S3 and Silver Sulfide have following formulas(given in attachments). Bismuth has valency (v or iii), Sulpher has (iv) and Silver has (i,ii) (a) Can Silver be doped in Bi2S3. (b) If doped, how...
11 July 2023 8,052 0 View
has anyone used ID2T toolkit which is used to inject synthetic attacks in given pcap file? Actually I am stuck at some place and I need help for my research Thankyou
06 February 2022 7,437 1 View
I want to know why extact by mask function changes or makes the x and y components same by default. I have used the function for different products raster files. Every time the extract by mask...
18 October 2021 4,245 12 View
Suggest some good gel analyzer software for free download. easy to use access and preferable for publications
11 August 2021 4,001 0 View
kindly explain the relative and residual activity of enzyme with formula
10 August 2021 7,959 5 View
Simply, i want to average out this data for my required temporal resolution. I am willing if anyone want further explanation.
13 July 2021 3,173 2 View
Hi every one. i am trying to write my thesis chapter about the important growth models influenced by public expenditure or government spending. Starting from Classical or Keynes, please guide...
18 May 2021 3,312 0 View
Can any one share the calculations. as double ammount of ammonia is present in ammonium sulphate. 0.1mM solution of ammonium sulphate contain 200 micromoles of ammonia is this correct. i have to...
29 December 2020 3,553 7 View
I am working on asparaginase producing bacteria. For whole genome sequencing of bacteiral strains I need miniresearch grants.
17 December 2020 3,781 2 View
I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...
15 July 2024 9,025 3 View
Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...
09 July 2024 5,508 0 View
I synthesize silver nanoparticles ,and reserve it in water. Then, when I sonicate it some minutes or longer ,it couldn't be dispersed, most of them aggregate .Otherwise ,when I sonicate several...
09 July 2024 7,335 0 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
HI , i am concerned about any resist hardening effect of Post bake my LOR +PPR coated sample @90C for 5 mins. I wanted to do liftoff in acetone but the metals are hard to peel off. I cant use PG...
18 June 2024 5,741 0 View
Does someone know any precipitating agent to precipitate glucose from 10 % glucose solution? or to remove glucose from solution
13 June 2024 438 0 View
Hello I have synthesized a dipeptide in an aqueous media. Now I want to precipitate and filter it. How to precipitate it. Thank you in advance. Velmurugan S
11 June 2024 525 0 View
Use a solution of acetone and water to perform an extraction. After performing it, evaporate the acetone and lyophilize the extracts, but I am looking for a methodology to confirm that acetone has...
10 June 2024 5,047 3 View
We are currently trying to replace acetate when dehyrdating ("drying") cryo sections on glass slides with a less harmful alternative. For many chemical purposes ethyle lactate is recommended - has...
10 June 2024 5,213 3 View
What is the easiest way to download daily precipitation data for CMORPH, PERSIAN, TRMM and CHIRPS?
02 June 2024 4,038 2 View