I am working on Asparaginase prouducing bacteria.is there any mini research grant upto 0.5 million available in pakistan for Phd scholar?

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How to perform IDS on real traffic using machine learning?

I'm working on IDS using machine learning on real traffic. basically, Lots of work has been done on datasets and I have found a very few works on testing on real traffic. So I want to know how can...

18 October 2020 2,485 3 View

What are protein-ligand interactions for each of the ligands?

Can someone please simulate and explain these protein-ligand interactions for each of the ligands: Please help me, it's urgent. Protein 5I08 : https://www.rcsb.org/structure/5I08 Ligands 1R42 :...

24 March 2020 4,144 4 View

Is it necessary to prepare FMO controls for each sample to be analyzed when there are two or more samples available?

Hi, I am analysing human peripheral blood by flow cytometry and I have to prepare FMO controls. If I have two or more samples from different persons then should I need to prepare FMO controls for...

27 October 2015 3,771 2 View

A lot are graduated,but only few get jobs,How to change the state of affairs and decrease this gap.

Researchers and Educationists,Don't you feel Its our duty to provide solutions and suggestions.

01 January 1970 4,250 3 View

Does Solar Photovoltaic system will really change the system of conventional generation in terms of cost of per unit of electricity and life?

Researchers kindly answer with statistics

01 January 1970 2,469 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View