I am working on Asparaginase prouducing bacteria.is there any mini research grant upto 0.5 million available in pakistan for Phd scholar?

More Ifrah Shafqat's questions See All

How many grams or milligrams of biosynthesized AgNPs were obtained after being totally dried?

In the methodology of biosynthesis of silver nanoparticles

15 July 2023 3,768 4 View

Can we dope Silver in Bismuth Sulfide..? What will be the method (via Solid state Method)??

Bi2S3 and Silver Sulfide have following formulas(given in attachments). Bismuth has valency (v or iii), Sulpher has (iv) and Silver has (i,ii) (a) Can Silver be doped in Bi2S3. (b) If doped, how...

11 July 2023 8,052 0 View

Has anyone have used ID2T toolkit which is used to inject synthetic attacks in your given dataset?

has anyone used ID2T toolkit which is used to inject synthetic attacks in given pcap file? Actually I am stuck at some place and I need help for my research Thankyou

06 February 2022 7,437 1 View

I have cell size (x, y)=(2.5, 2) spatial resolution of a raster file. When i use extract by mask function in arcgis then the resolution changes?

I want to know why extact by mask function changes or makes the x and y components same by default. I have used the function for different products raster files. Every time the extract by mask...

18 October 2021 4,245 12 View

Can you please gel analyzer software free download???

Suggest some good gel analyzer software for free download. easy to use access and preferable for publications

11 August 2021 4,001 0 View

What is the difference between% residual and relative activity of enzyme and what is exact formula for both?

kindly explain the relative and residual activity of enzyme with formula

10 August 2021 7,959 5 View

I have a heavy 2Gb .dat.nc file, this nc file has multidimentional data. Now i want to separate each time slice file for this multidimentional data?

Simply, i want to average out this data for my required temporal resolution. I am willing if anyone want further explanation.

13 July 2021 3,173 2 View

The chronological classification of public expenditure led economic growth models?

Hi every one. i am trying to write my thesis chapter about the important growth models influenced by public expenditure or government spending. Starting from Classical or Keynes, please guide...

18 May 2021 3,312 0 View

Can we reuse acetone that is used in acetone precipitation of proteins??

I am doing acetone precipitation for partial purification of asparaginase from bacterial strains. Can I reuse acetone for the same purpose for the same strain. As it is used in bulk quantity for...

17 January 2021 1,137 0 View

How many micromoles of ammonia are there in 0.1mM solution of ammonium sulphate???

Can any one share the calculations. as double ammount of ammonia is present in ammonium sulphate. 0.1mM solution of ammonium sulphate contain 200 micromoles of ammonia is this correct. i have to...

29 December 2020 3,553 7 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Can you visualize platelets using EVOS ?

Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...

24 July 2024 9,337 3 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View