Hello All,
Is it possible to re-developing signal without stripping in Western blot? and How do you do it? Please share. It has been stuck in my head for a while. My objective is just to re-develop signal the same protein on the membrane again, in case ECL expired or want to capture image again. No need to change primary and secondary antibody.
From what I read and heard, there are three ways to re-developing signal in western blot:
1. Stripping, blocking, and then re-probing primary and secondary antibodies again, then re-developing with ECL -> lost some protein sample. This method is written in every protocol.
2. Don't strip, just re-probing with secondary antibody, then re-developing with ECL.
3. Washing membrane in TBST for a few time, then re-developing with ECL. -> heard from lab mate -> haven't found reference in google.
Have you ever tried 2nd and 3rd method? Does it work? Please verify
Thank you very much,