Ramin Bahmani

2 Questions 8 Answers 0 Followers

Questions related from Ramin Bahmani

What are the important tips for amplification of a large fragment by PCR?

I am trying to amplify a large DNA fragment (about 5 Kb) by PCR. I used a FX-PCR master mix (for long and fidelity PCR). The PCR condition is as follow: 95C 5 min, 35 cycles of ; 95C 30 sec, 53C...

19 August 2016 9,930 14 View

Can anyone help with a smear of the digestion products on agarose gel?

22 July 2014 9,778 4 View