Does anyone have the Wiley Journal word Template?

Hi I need a word template file of Wiley online library because it is not available on their website.

27 December 2020 7,208 4 View

Advice on choosing FISH probes with good sensitivity for lncRNA?

Hi, I am doing localization and abundance studies on a novel lncRNA. I would appreciate some advice on the kind of FISH or LNA tech. kit/fluorophore to use and the products which have high...

18 October 2020 7,701 2 View

What is the application of 2 photon absorption property of a material?

what are devices that use the 2 photon absorption property of a material

03 October 2020 9,938 2 View

How to know which Metal center is more electron Rich in Organometallic complexes by DFT?

27 July 2020 2,679 3 View

Are you interested to review for Civil Engineering Research Articles and Latest Information?

Dear Sir/Madam, The following link is related to the "Civil Engineering Publication Reviewers Registration Form". If you are interested to review the Research Articles, Book Chapters, Conference...

23 July 2020 4,068 3 View

What could be possible reasons for this type of cracking ?

Recently came across a case where a CC slab (micro-alloyed peritectic grade) has broken into two pieces. The slab broke right from the middle into two separate pieces when it was dropped. What...

29 May 2020 8,882 1 View

Using FLUENT UDF to find the location of a point iso-surface ?

Is there a way to access a point iso-surface in UDF and find its co-ordinate. I need to calculate velocity based on the movement of this point over time

25 May 2020 5,700 4 View

What is the Physical meaning of wiberg bond Indices?

I am trying to understand the physical meaning of Wiberg bond indices, how does the value of WBI explain the interaction between any atoms? For example, I have two Ag atoms in one of my structure...

24 April 2020 2,067 4 View

Help with the identification of an XRD peak?

Hi everyone, I have a sample of bioash (produced from biosolids by heating in an oven at 550 deg centigrade - air environment). I have carried out x-ray fluoresence as well to identify the...

15 April 2020 6,205 3 View

What is the current evidence on the role of topical metronidazole in the management of periodontal and peri-implant diseases?

Metronidazole is an imidazole known to be most active against obligate anaerobes. So if we apply this principle to periodontal diseases, we should use it only at the base of deep periodontal...

25 February 2020 3,926 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View