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Questions related from Rajeev Kungur Brahma
I am currently working on several proteins with at least 4 disulfide bonds. I am isolating inclusion bodies and refolding the proteins. I would like to try expression of the same using CyDisCo...
22 April 2019 1,525 3 View
We are trying to isolate DNA from liver tissue of snake preserved in alcohol/RNAlater using phenol:chloroform method. However, we are not getting good quality DNA. There is smearing or trailing of...
31 December 2013 1,582 10 View
I have cloned a cDNA coding for 7 kDa cysteine-less protein into pET20b which has leader sequence. The recombinant vector was sequenced using T7 promoter and the reading frame of the protein was...
26 December 2013 7,045 7 View
I use 18% tris-tricine SDS-PAGE gel (ethylene glycol added) for separating protein samples. I use 0.1M Tris, 0.1M tricine, 0.4% SDS buffer for running the gel. I run the gel at constant voltage of...
21 November 2013 9,620 1 View
We are trying to clone a small protein of about 7 kDa into pET20b or pET22b which has a N-terminus His-tag and express the same in E. coli BL21(DE3)pLysS. Will the His-tag affect the structure or...
23 September 2013 5,854 27 View
We have synthesized cDNA using Clontech cDNA construction kit. The kit protocol mentions purification of cDNA by phenol:chloroform method after proteinase K digestion. Can this be done using...
07 September 2013 8,008 4 View
We are trying to prepare fatty acid methyl esters of rhamnolipids for GC-MS analysis. We have used one method from a research article, but we faced difficulties with the method as high temperature...
28 August 2013 1,515 3 View
28 August 2013 9,233 16 View
It is seen that generally people sequence 16S rDNA for the identification of a bacterium. However, it has low phylogenetic power at the species level (Janda and Abbott, 2007) and cannot be used to...
21 March 2013 9,490 89 View
I have installed Biolinux 6 based on ubuntu 10.04. However, sound is not working even after installation of alsa utilis, alsamixergui and adding of "options snd-hda-intel probe_mask=1 model=hp"...
17 November 2012 4,134 5 View
I am working on tick salivary gland proteins and need to describe the tick in my present study. What morphological characters are essential for the identification of ticks? I would also like to do...
17 August 2012 8,261 16 View
