I want to prepare benzofused pyrazole compounds using isatin hydrazine and some other compounds but i can not get single spot on TLC.
How to enhanced h-index in Research Gate?
04 August 2024 3,368 4 View
why uv is done and why photocatalytic measurement is done although both use uv radiation
09 June 2024 8,750 1 View
it would be helpful if you provide the reference to your answer
11 May 2024 5,989 4 View
I just completed my M.Sc Physics while researching on ferrites in Photocatalytic property. Now i want to do My Phd does anyone suggest me any topic or any good university for doing my reserch
19 April 2024 3,772 2 View
Hello, RG Members, I have designed the COBRA primers using the UCSC genome browser for the promoter regions of genes of my intreset. are these primers reliable? I have tried the other softwares...
17 April 2024 7,882 0 View
I'm working on sugars present in honey sample.I tried paper and TLC with different mobile phases like Butanol:Glacial Acetic acid:Water and Butanol:Pyridine:Water but I'm not getting expected...
05 March 2024 9,609 1 View
I'm working on utilization of wastes for biogas production.I streak a loopful cowdung slurry collected from biogas plant on sodium thioglycolate media.I got some colonies on the media.I need to...
28 February 2024 1,877 4 View
Can anyone knows about how to find the restriction sites using rs IDs. Are there any tools to find?
21 February 2024 2,794 0 View
I'm trying to seprate amino acids using silica gel.When I spray ninhydrin solution on silica gel it get disturbed.Can anyone tell me how to observe separated amino acid on silica gel plate?
06 February 2024 8,366 2 View
Hello everyone, This is my first question and very curious to learn why my GST fused protein shows band in the cell Lysate flow through, lysis buffer, wash buffer, but absolutely no bands on the...
25 January 2024 7,470 3 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
How can I measure the thickness of thin film by Uv-Vis?
06 August 2024 7,810 3 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View
I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?
31 July 2024 8,415 1 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View