I have calculated the encapsulation efficiency of a protein in liposomes and quantified it using bradford assay. There are 4 groups to compare and each group has 3 samples in it. Each sample has triplicate values.
Of course.
We need to calculate the encapsulation efficiency of magnetite nanoparticles in liposomes.
07 August 2019 8,529 3 View
We prepared 3 types of uncoated liposomes (3um) with different lipid to cholesterol ratios: 1:4 cholesterol to lecithin 1:6 1:8 Diluent: serum Core: water Incubation time: 3 days Temperature :...
07 August 2019 9,046 7 View
Is it possible that the protein travels or crosses the sample loading dye front?
04 May 2019 5,448 1 View
I am receiving bands between 10 to 15kda of hemoglobin on SDS PAGE. Each subunit of hemoglobin is 16kda. What should be the problem? 12% resolving gel 4% Stacking gel.
03 April 2019 239 4 View
I want to stain liposome with rhodamine. For this I need protocol.
03 April 2019 6,889 1 View
I want to know the absorbance peak range of UV Vis spectrum of fe3o4 nanoparticles
02 March 2019 2,950 4 View
I need pure hemoglobin variant A.
31 December 2018 6,988 4 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
How to manually calculate p value in ANOVA? what is the equation or formula for it?
03 March 2021 4,928 5 View
I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...
01 March 2021 3,703 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...
27 February 2021 9,626 4 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...
24 February 2021 7,122 1 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
While testing assumptions, Shapiro-Wilk Test failed. However, I proceeded with the analysis and the interaction effect was significant. During our course- lectures, it was mentioned that Anova...
24 February 2021 228 3 View
How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...
24 February 2021 166 6 View