Is it possible that the protein travels faster than the sample loading dye?

05 May 2019 1 5K Report

Is it possible that the protein travels or crosses the sample loading dye front?

It is not likely. The protein might travel at the same rate as the loading dye because it is too small to be resolved on the gel. Try a higher acrylamide concentration.

Badges
Science topic

More Saleha Hafeez's questions See All

How can we calculate the encapsulation efficiency of nanoparticles in liposomes?

We need to calculate the encapsulation efficiency of magnetite nanoparticles in liposomes.

07 August 2019 8,529 3 View

Why the size of uncoated liposomes decreases with the passage of time?

We prepared 3 types of uncoated liposomes (3um) with different lipid to cholesterol ratios: 1:4 cholesterol to lecithin 1:6 1:8 Diluent: serum Core: water Incubation time: 3 days Temperature :...

07 August 2019 9,046 7 View

Can we perform one way ANOVA on 4 groups each with triplicates?

I have calculated the encapsulation efficiency of a protein in liposomes and quantified it using bradford assay. There are 4 groups to compare and each group has 3 samples in it. Each sample has...

06 July 2019 8,489 1 View

SDS-PAGE of Hemoglobin?

I am receiving bands between 10 to 15kda of hemoglobin on SDS PAGE. Each subunit of hemoglobin is 16kda. What should be the problem? 12% resolving gel 4% Stacking gel.

03 April 2019 239 4 View

How can we stain liposomes with rhodamine for fluorescent microscopy?

I want to stain liposome with rhodamine. For this I need protocol.

03 April 2019 6,889 1 View

What is UV VIS peak range of Magnetite nanoparticles?

I want to know the absorbance peak range of UV Vis spectrum of fe3o4 nanoparticles

02 March 2019 2,950 4 View

Is there an alternative way to purify hemoglobin variant A from other hemoglobin variants other than HPLC?

I need pure hemoglobin variant A.

31 December 2018 6,988 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

How can I label an antibody to determine how far it is travelling from the microdialysis probe?

I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of...

09 February 2021 3,981 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View