Is it possible that the protein travels or crosses the sample loading dye front?
It is not likely. The protein might travel at the same rate as the loading dye because it is too small to be resolved on the gel. Try a higher acrylamide concentration.
We prepared 3 types of uncoated liposomes (3um) with different lipid to cholesterol ratios: 1:4 cholesterol to lecithin 1:6 1:8 Diluent: serum Core: water Incubation time: 3 days Temperature :...
07 August 2019 9,151 7 View
We need to calculate the encapsulation efficiency of magnetite nanoparticles in liposomes.
07 August 2019 8,602 3 View
I have calculated the encapsulation efficiency of a protein in liposomes and quantified it using bradford assay. There are 4 groups to compare and each group has 3 samples in it. Each sample has...
06 July 2019 8,537 1 View
I am receiving bands between 10 to 15kda of hemoglobin on SDS PAGE. Each subunit of hemoglobin is 16kda. What should be the problem? 12% resolving gel 4% Stacking gel.
03 April 2019 360 4 View
I want to stain liposome with rhodamine. For this I need protocol.
03 April 2019 6,971 1 View
I want to know the absorbance peak range of UV Vis spectrum of fe3o4 nanoparticles
02 March 2019 3,000 4 View
I need pure hemoglobin variant A.
31 December 2018 7,052 4 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...
19 July 2024 4,868 1 View
These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..
13 July 2024 9,701 1 View
This is the code I have. I would need: An initial configuration (position and momentum), the orientation of the ecliptic with respect to the direction of travel by the sun, The orientation of the...
09 July 2024 6,063 2 View
The harmful Dye pollution from industrial processes such as textile generated how to remove with magnetic biochar ?
07 July 2024 8,118 1 View
500 ng RNA is converted to cDNA to be genetically expressed by qpcr sybergreen dye, 1 ul primer F, 1 ul primer R, 10 ul master mix, 1 ul cdna template, and instead of a complete total volume...
06 July 2024 5,478 4 View
Assessing the Feasibility of Vacation Homes and Vehicle Rentals in India In my article, "Assessing the Feasibility of Vacation Homes and Vehicle Rentals in India: Understanding Market Needs,...
03 July 2024 5,535 1 View
Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...
30 June 2024 9,300 0 View
I seek to understand the theoretical basis for selecting the optimal temperature for the thermal regeneration process of exhausted adsorbents, such as activated carbon loaded with dye molecules....
28 June 2024 1,867 3 View