I want to stain liposome with rhodamine. For this I need protocol.
Look into Thunnissen IE, Marani E, Rietveld WJ Homing of Lucifer Yellow liposomes into hypothalamic neurons. J Neurosci methods 1984, 12: 113-123.
We need to calculate the encapsulation efficiency of magnetite nanoparticles in liposomes.
07 August 2019 8,529 3 View
We prepared 3 types of uncoated liposomes (3um) with different lipid to cholesterol ratios: 1:4 cholesterol to lecithin 1:6 1:8 Diluent: serum Core: water Incubation time: 3 days Temperature :...
07 August 2019 9,046 7 View
I have calculated the encapsulation efficiency of a protein in liposomes and quantified it using bradford assay. There are 4 groups to compare and each group has 3 samples in it. Each sample has...
06 July 2019 8,489 1 View
Is it possible that the protein travels or crosses the sample loading dye front?
04 May 2019 5,448 1 View
I am receiving bands between 10 to 15kda of hemoglobin on SDS PAGE. Each subunit of hemoglobin is 16kda. What should be the problem? 12% resolving gel 4% Stacking gel.
03 April 2019 239 4 View
I want to know the absorbance peak range of UV Vis spectrum of fe3o4 nanoparticles
02 March 2019 2,950 4 View
I need pure hemoglobin variant A.
31 December 2018 6,988 4 View
Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru
02 March 2021 1,787 2 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...
01 March 2021 3,052 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...
26 February 2021 8,399 5 View