How can we stain liposomes with rhodamine for fluorescent microscopy?

04 April 2019 1 7K Report

I want to stain liposome with rhodamine. For this I need protocol.

Look into Thunnissen IE, Marani E, Rietveld WJ Homing of Lucifer Yellow liposomes into hypothalamic neurons. J Neurosci methods 1984, 12: 113-123.

Badges
Science method

More Saleha Hafeez's questions See All

Why the size of uncoated liposomes decreases with the passage of time?

We prepared 3 types of uncoated liposomes (3um) with different lipid to cholesterol ratios: 1:4 cholesterol to lecithin 1:6 1:8 Diluent: serum Core: water Incubation time: 3 days Temperature :...

07 August 2019 9,151 7 View

How can we calculate the encapsulation efficiency of nanoparticles in liposomes?

We need to calculate the encapsulation efficiency of magnetite nanoparticles in liposomes.

07 August 2019 8,602 3 View

Can we perform one way ANOVA on 4 groups each with triplicates?

I have calculated the encapsulation efficiency of a protein in liposomes and quantified it using bradford assay. There are 4 groups to compare and each group has 3 samples in it. Each sample has...

06 July 2019 8,537 1 View

Is it possible that the protein travels faster than the sample loading dye?

Is it possible that the protein travels or crosses the sample loading dye front?

04 May 2019 5,511 1 View

SDS-PAGE of Hemoglobin?

I am receiving bands between 10 to 15kda of hemoglobin on SDS PAGE. Each subunit of hemoglobin is 16kda. What should be the problem? 12% resolving gel 4% Stacking gel.

03 April 2019 360 4 View

What is UV VIS peak range of Magnetite nanoparticles?

I want to know the absorbance peak range of UV Vis spectrum of fe3o4 nanoparticles

02 March 2019 3,000 4 View

Is there an alternative way to purify hemoglobin variant A from other hemoglobin variants other than HPLC?

I need pure hemoglobin variant A.

31 December 2018 7,052 4 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View