Should be feasible without problem. There's a good protocol in Harlowe & Lane, Using Antibodies.
This not a trivial problem. You need to know, how the antibody was generated. If you use a different protocol for a hapten, such as tetracyclines, your HRP conjugate might not work at all.
thanks Michael for your reply, but for example if i want to label tetracyclines with HRP ? like as previously it has been documented that Penicillin is labeled with HRP ?
The labelling of penicillin with HRP is even more difficult since most penicillins (and the respective penicillin conjugates) slowly degrade in water. In the case of tetracyclines it is definitely possible to make stable conjugates, but they may not work in your ELISA, because of a wrong structure. For an ELISA you need essentially the same structure for the enzyme conjugate as for the immunogen, which generated the antibody. Do you have an antibody already?
Perhaps I misunderstood your question. Do you want to label your analyte (drug) directly in the sample to detect it enzymatically?
Unfortunately, this will not work. All labeling chemistries are more or less non-selective, which means that nearly all compounds will be labeled, not only your analyte.
Sorry, I just noticed that I have misread "antibiotics" as "antibodies", so it's not as straightforward and trivial as I have assumed. You need to derivatize the tetracycline in a way that you introduce a spacer that ends with an amino group to render it accessible to labeling with HRP the "standard" way (periodate oxidation, the coupling, followed by cyanoborohydride reduction).
Interesting ,discussion, knowledge enhancing for new entrants in the field
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