We regularly use cell strainers for splenocyte preparations, but at $150/lot per pre-clinical trial for something that just basically just strains spleen casings- I am wondering...does anyone else prep spleen cells without them and without running into trouble of cells clumping (for cell assays or flow cyotmetry) or possibly have an alternative option (e.g. fine, sterilized tulle)?
To save money we reuse the strainers as follows:
1. Remove as much debris from the strainer (careful not to puncture the screen)
2. Place strainers in a warm 0.1% SDS solution and soak overnight.
3. Next day rinse strainers with copious amounts of water (very important to remove all residual SDS).
4. store strainers in 70% ETOH until use.
We find the stainers can be reused at least 3-times.
Hi...I have prepared murine splenocyte suspension by using homogenizer for various assays. Have a look at attached paper. Hope u get some idea.
Article Deltamethrin-Induced Oxidative Stress and Mitochondrial Casp...
To save money we reuse the strainers as follows:
1. Remove as much debris from the strainer (careful not to puncture the screen)
2. Place strainers in a warm 0.1% SDS solution and soak overnight.
3. Next day rinse strainers with copious amounts of water (very important to remove all residual SDS).
4. store strainers in 70% ETOH until use.
We find the stainers can be reused at least 3-times.
You could use nylon mesh cloth from Tetko Inc, NY, USA of requisite pore size but check whether it is autoclavable
In continuation to above - yes the Tetko cloth mesh is also autoclavable so it can be used for filtration of splenocytes
Dear Jennifer Lynn Bath,
I'd like to ask the same question, i found your question with answers, i hope to find as soon as possible nylon Filter. Thank you all for your contribution.
Thank you for the input everyone. We found this helpful as well Hadjer. We used the mesh nylon recommendation to actually purchase several yards of tulle from a local store for about $2.00. It was autoclavable and worked great. Thanks!
@ Daniel J. Medina
Would you be able to provide some more details about your protocol ?
1- do you remove debris by rubbing the mesh with a pippet tip ?
2- what should be the temperature of SDS solution ? also, is there a need to perform this step under agitation ?
Thank you in advance !
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