How to dissolve Pentachloronitrobenzene?

Pentachloronitrobenzene is used to prepare the "Nash & Snyder" media to isolate the Fusarium. But this is not dissolving in 75% ethanol and distilled water (Normal and hot both). So Please...

05 February 2021 687 1 View

How monolayer graphene can be grown on copper foil via LPCVD technique?

Please tell me about the rate (in SCCM unit) of the flow of gases and pressure inside the tube.

12 October 2020 8,315 3 View

If we cross Diploid plant (Female Parent) x Tetraploid plant. How much chances of getting viable seed?

In case of guava crossing of Diploid (Psidium guajava, 2n = 22) with P. Molle (2n=44). Is there any chance to get viable seed set?

16 June 2020 9,317 3 View

Is there any mathematical model to study the electric fringe field for parallel plates having equal and opposite potential?

Some Mathematical expressions will be helpful.

26 April 2020 8,000 2 View

Which form of polyaniline (emeraldine base or emeraldine salt ) show antibacterial property and why?

The emeraldine form of polyaniline often refer to as emeraldine Base (EB) is neutral, if it is doped (protonated) with acid(1M HCl) it is called emeraldine salts (ES) is conducting, with imine...

24 February 2020 5,117 3 View

Is there need to downscale CORDEX (RCM) data for our region? or Can we directly apply them after bias correcting them?

I have a query regarding data collected from CORDEX models and CMIP5 models. After reading about both, I have concluded that data from CMIP5 have to statistical downscale first then can apply for...

22 December 2019 2,655 5 View

How to perform a meta-analysis for single group?

How to perform a meta-analysis for single group? Single group means you don't have a comparison group. You have estimates i.e. Mean+_SD with sample size OR events and sample size for one...

11 August 2019 5,790 14 View

Institutional investors may or may not trade every day then how can we treat the data to arrange time series?

Hello, Institutional investors may or may not buy or sell the stock every day then how can we treat the data to arrange the time series. kindly give your suggestions.

31 July 2019 4,568 3 View

What parameters to take into consideration while trying to minimize pressure drop and maximize heat transfer across an enclosed diesel generator?

I am trying to use Genetic Algorithm for a Pareto Optimal solution for minimizing pressure drop and maximizing heat transfer across a diesel generator. I am not sure about which parameters to...

30 October 2018 8,482 1 View

Is there available the standard BMI criteria for RATS to assessment of normal wt., Overweight, obesity of rats???

If there is any standard BMI criteria to assess wt. To categories in normal wt.,Ower wt. and obesity of Rats

20 August 2018 5,824 1 View

No title

03 March 2021 5,360 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View