I am trying to calculate the reference gene expression stability for my qPCR assay since nobody has worked in case of my soybean.
I am working on relative quantification of a fungus inside soybean tissue.
Does anybody have clue on this?
I am trying to do qPCR using some primers. The primers are specific for lower concentrations. However, I do not see any amplifications at higher concentrations. What could be the reason for...
06 July 2019 4,231 1 View
The DNA that is in my sample is less to be detected as compared to host DNA. So, I have to increase my primers concentration from 10 micromolar to more. So, was wondering how much concentration...
01 January 1970 4,757 4 View
Can anyone explain how it works. The fungus lives in the vascular bundles.
01 January 1970 7,606 2 View
I am a beginner in experimental design so maybe I am not understanding the basics. In a class that I took , for a given population and confidence level and interval, the recommended sample size is...
01 January 1970 966 0 View
Hello Everyone, I am using DNeasy mini protocol to extract DNA from at least 7 days old culture in PDA. When I tried it, several times I am getting less than 10 ng/micro liter of DNA. It is...
01 January 1970 1,429 2 View
I am working on a fungus and was wondering if I can extract DNA from the pure culture of the fungus and measure the concentration of DNA and do a serial dilution for absolute...
01 January 1970 6,588 4 View
I am doing real time PCR , relative quantification for a fungus that infects soybean. What housekeeping genes do I use for this? Are these for the fungus itself? I did not find them for my fungus...
01 January 1970 970 1 View
I am trying to replicate using a set of primers mentioned in a paper Quantification of Fusarium solani f. sp. glycines isolates i... The thermal cycling conditions mentioned are 95 degree...
01 January 1970 1,521 9 View
I am analyzing the process of colonization by a fungus on plant stem. I am trying to do real time PCR for this process. In the meantime I am looking for papers, I am finding different processes...
01 January 1970 9,372 4 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...
06 August 2024 1,896 1 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...
26 July 2024 6,520 1 View
Am trying to develop a lesson/lab to determine and compare antioxidant properties of soybeans of various colors. Preference would be low tech and low cost. Any assistance is greatly appreciated.
25 July 2024 7,498 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View