Bhawana Ghimire

10 Questions 12 Answers 0 Followers

Questions related from Bhawana Ghimire

The primers that I have are amplifying lower concentration of DNA but not higher. Is this even possible? What can be done to fix this?

I am trying to do qPCR using some primers. The primers are specific for lower concentrations. However, I do not see any amplifications at higher concentrations. What could be the reason for...

07 July 2019 4,228 1 View

Why are most greenhouse studies having small population and sample sizes as compared to the ones described in statistical books?

I am a beginner in experimental design so maybe I am not understanding the basics. In a class that I took , for a given population and confidence level and interval, the recommended sample size is...

01 January 1970 962 0 View

Trying to replicate the research paper , but the thermal cycling conditions of the primers do not work. Why?

I am trying to replicate using a set of primers mentioned in a paper Quantification of Fusarium solani f. sp. glycines isolates i... The thermal cycling conditions mentioned are 95 degree...

01 January 1970 1,517 9 View

How much concentration of primers is ideal to use for sensitivity?

The DNA that is in my sample is less to be detected as compared to host DNA. So, I have to increase my primers concentration from 10 micromolar to more. So, was wondering how much concentration...

01 January 1970 4,754 4 View

What is the usual concentration of Diaporthe fungus using DNeasy mini kit protocol?

Hello Everyone, I am using DNeasy mini protocol to extract DNA from at least 7 days old culture in PDA. When I tried it, several times I am getting less than 10 ng/micro liter of DNA. It is...

01 January 1970 1,423 2 View

I am trying to extract DNA of fungus from plant tissue and amplify the fungal DNA. Any suggestions on the protocol?

Can anyone explain how it works. The fungus lives in the vascular bundles.

01 January 1970 7,602 2 View

Can I prepare standard curve from DNA extracted from pure culture of my fungus for absolute quantification?

I am working on a fungus and was wondering if I can extract DNA from the pure culture of the fungus and measure the concentration of DNA and do a serial dilution for absolute...

01 January 1970 6,584 4 View

What is the best protocol for qPCR of fungal tissue?

I am analyzing the process of colonization by a fungus on plant stem. I am trying to do real time PCR for this process. In the meantime I am looking for papers, I am finding different processes...

01 January 1970 9,366 4 View

The housekeeping gene stability analysis?

I am doing real time PCR , relative quantification for a fungus that infects soybean. What housekeeping genes do I use for this? Are these for the fungus itself? I did not find them for my fungus...

01 January 1970 966 1 View

Can we analyse the reference gene expression without software mentioned in literature like Normgne, REST etc?

I am trying to calculate the reference gene expression stability for my qPCR assay since nobody has worked in case of my soybean. I am working on relative quantification of a fungus inside soybean...

01 January 1970 593 0 View