The primers that I have are amplifying lower concentration of DNA but not higher. Is this even possible? What can be done to fix this?

I am trying to do qPCR using some primers. The primers are specific for lower concentrations. However, I do not see any amplifications at higher concentrations. What could be the reason for...

06 July 2019 4,201 1 View

How much concentration of primers is ideal to use for sensitivity?

The DNA that is in my sample is less to be detected as compared to host DNA. So, I have to increase my primers concentration from 10 micromolar to more. So, was wondering how much concentration...

01 January 1970 4,725 4 View

What is the usual concentration of Diaporthe fungus using DNeasy mini kit protocol?

Hello Everyone, I am using DNeasy mini protocol to extract DNA from at least 7 days old culture in PDA. When I tried it, several times I am getting less than 10 ng/micro liter of DNA. It is...

01 January 1970 1,393 2 View

I am trying to extract DNA of fungus from plant tissue and amplify the fungal DNA. Any suggestions on the protocol?

Can anyone explain how it works. The fungus lives in the vascular bundles.

01 January 1970 7,572 2 View

The housekeeping gene stability analysis?

I am doing real time PCR , relative quantification for a fungus that infects soybean. What housekeeping genes do I use for this? Are these for the fungus itself? I did not find them for my fungus...

01 January 1970 938 1 View

Can I prepare standard curve from DNA extracted from pure culture of my fungus for absolute quantification?

I am working on a fungus and was wondering if I can extract DNA from the pure culture of the fungus and measure the concentration of DNA and do a serial dilution for absolute...

01 January 1970 6,554 4 View

What is the best protocol for qPCR of fungal tissue?

I am analyzing the process of colonization by a fungus on plant stem. I am trying to do real time PCR for this process. In the meantime I am looking for papers, I am finding different processes...

01 January 1970 9,337 4 View

Can we analyse the reference gene expression without software mentioned in literature like Normgne, REST etc?

I am trying to calculate the reference gene expression stability for my qPCR assay since nobody has worked in case of my soybean. I am working on relative quantification of a fungus inside soybean...

01 January 1970 562 0 View

Trying to replicate the research paper , but the thermal cycling conditions of the primers do not work. Why?

I am trying to replicate using a set of primers mentioned in a paper Quantification of Fusarium solani f. sp. glycines isolates i... The thermal cycling conditions mentioned are 95 degree...

01 January 1970 1,488 9 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View