Balakumaran Sathiyamani

3 Questions 1 Answers 0 Followers

Questions related from Balakumaran Sathiyamani

Signal peptide or fusion partners like thioredoxin for hydrophobic protein expression in E.Coli?

I am trying to express HIV1 antisense protein in E.Coli. The protein is hydrophobic and also has two N-terminal cysteine triplets. I am planning to add a pelb signal peptide for periplasmic...

01 February 2024 1,828 1 View

Can we add recombinant enterokinase to the column directly for on-column tag removal?

I am using cobalt column for purification. If anyone has done it, kindly share the protocol.

25 January 2024 2,909 0 View

What would be the approximate purified protein yield from HEK 293t cells?

Plasmid Concentration - 25ug per t75 flask Cell number - 10 million cells Protein size - 25 KDa Purification procedure - His tag purification using cobalt resin. I am going to use this purified...

16 November 2023 6,985 1 View