When protein degrading enzymes present with intact virus in water samples, is it possible to degrade the capsid protein of those virus by those proteases (Especially for the commercially available proteases such as Proteinase K).
Yes, but you have make sure your capsid can be digested with a certain protease.
For our purpose (release of the viral genome) we digest AAV capsids at 56°C for 2 h (Proteinase K)
Table from: Van Vliet et al., "The Role of the Adeno-Associated Virus
Capsid in Gene Transfer", (2008), Methods Mol Biol 437: 51-91.
Dear Mario, thanks for the informative answer.
I would like to clarify something In the given reference table: the - sign (for example AAV1, with trypsin or proteinase K), implies that those mentioned enzymes could not affect the infectivity of AAV1? Am I correct?
Thanks agin.
You're welcome.
Exactly. The data of the table is based on the incubation of intact, infectious particles with the indicated enzymes.
It has been shown that AAV1 or AAV5 loose less than 1 log of infectivity following 24 h of digestion with trypsin whereas AAV2 looses 3-4 log of infectivity at the same time.
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