What is the accepted recovery rate range for an acid digestion(HCl:HNO3), followed by an AES analysis?

I'm doing a heavy metal analysis by digesting bird feathers using acids. And in order to verify whether the method i use and the instrument i use give accurate results i was planning to do a...

22 September 2020 4,129 3 View

If I dissolve 35 g of NaCl in 100 ml, what will be the approximate specific gravity?

If I dissolve 35 g of NaCl in 100 ml, what will be the approximate specific gravity?. Is it 1.35? or is there some trick? I read that saturated NaCl soltion has specific gravity of 1.2. Also...

13 May 2020 5,065 2 View

We all want to get back to Normalcy, do we want to get back to the same 'Normalcy'?

Before the Covid19 pandemic wrought havoc in all parts of the world, we were going at a rate. The popular notion that happiness or human well-being is directly associated with more and more one...

09 May 2020 8,694 13 View

How to avoid uneven (intensity) bands during Western Blotting?

I've uneven bands (size, intensity, and staining) when doing Western Blotting and I'm wondering what the problem could be. The protocol: -10% separating gel (0.375M Tris pH8.8; 0.1% SDS; 10%...

14 July 2019 6,577 8 View

How to ask a good question in RG? and get many responses?

Do we need to be specific? Sometime I feel that if we become specific with more details when we ask the questions, we loose some potential researchers from other fields. So the number of answers...

27 May 2019 3,627 24 View

Thai Electricity Production percentages (Breakdown according to source, for example Hydropower%, Thermal power%, Solar% etc.)?

I would like to know the, Thai Electricity Production percentages (Breakdown according to source, for example Hydropower%, Thermal power%, Solar% etc.)?

27 February 2019 9,882 6 View

Hello.. Is it possible to analyse the cross sectional area propagation of a flame by using mesh free method ?

18 January 2017 9,363 1 View

How to dissolve PEI (polyethylenimine)?

20 April 2016 2,880 22 View

Is there any possibility that tofu protein recovery can achieve more than 100%?

I make tofu from vegetable soybean (edamame). Water to bean ratio for tofu making is 1:12 and CaSO4.2H2O as coagulants. Vegetable soybean has moisture content 64% (wet basis) and protein 5% (wet...

16 October 2015 3,816 12 View

Could someone help me with some Neuronal Tissue Co-IP issues?

I have been doing co-immunoprecipitations for a while now using my own buffers (CHAPS lysis buffer + protease & phosphatase inhibitors) and Dynabeads protein G beads. Useful information: -The...

21 January 2014 5,209 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Which are the journals for free paper publication?

A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid

25 February 2021 9,790 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Can anyone recommend which is the best free questionnaire software please?

Hello, I am looking to send out links to an online survey. My previous research project used a paid for survey software. I am now looking for something that's free but straight forward to...

19 February 2021 5,641 3 View