What is the accepted recovery rate range for an acid digestion(HCl:HNO3), followed by an AES analysis?

I'm doing a heavy metal analysis by digesting bird feathers using acids. And in order to verify whether the method i use and the instrument i use give accurate results i was planning to do a...

22 September 2020 4,129 3 View

If I dissolve 35 g of NaCl in 100 ml, what will be the approximate specific gravity?

If I dissolve 35 g of NaCl in 100 ml, what will be the approximate specific gravity?. Is it 1.35? or is there some trick? I read that saturated NaCl soltion has specific gravity of 1.2. Also...

13 May 2020 5,065 2 View

How to avoid uneven (intensity) bands during Western Blotting?

I've uneven bands (size, intensity, and staining) when doing Western Blotting and I'm wondering what the problem could be. The protocol: -10% separating gel (0.375M Tris pH8.8; 0.1% SDS; 10%...

14 July 2019 6,577 8 View

How to ask a good question in RG? and get many responses?

Do we need to be specific? Sometime I feel that if we become specific with more details when we ask the questions, we loose some potential researchers from other fields. So the number of answers...

27 May 2019 3,627 24 View

Thai Electricity Production percentages (Breakdown according to source, for example Hydropower%, Thermal power%, Solar% etc.)?

I would like to know the, Thai Electricity Production percentages (Breakdown according to source, for example Hydropower%, Thermal power%, Solar% etc.)?

27 February 2019 9,882 6 View

Hello.. Is it possible to analyse the cross sectional area propagation of a flame by using mesh free method ?

18 January 2017 9,363 1 View

How to dissolve PEI (polyethylenimine)?

20 April 2016 2,880 22 View

Is there any possibility that tofu protein recovery can achieve more than 100%?

I make tofu from vegetable soybean (edamame). Water to bean ratio for tofu making is 1:12 and CaSO4.2H2O as coagulants. Vegetable soybean has moisture content 64% (wet basis) and protein 5% (wet...

16 October 2015 3,816 12 View

Could someone help me with some Neuronal Tissue Co-IP issues?

I have been doing co-immunoprecipitations for a while now using my own buffers (CHAPS lysis buffer + protease & phosphatase inhibitors) and Dynabeads protein G beads. Useful information: -The...

21 January 2014 5,209 3 View

Evaluation of extraction socket healing

How to evaluate extraction socket healing (when the wound is primarily sutured)? Most of the studies use Landry and Turnbull Healing Index. There is also the H2O2 bubbling test. Do you have any...

01 January 1970 2,794 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

Is this grounds for theophylline toxicity even though her plasma is within the therapeutic range? if it is, how is that possible?

A patient was given 300 mg Theophylline taken twice a day for the treatment of her bronchial asthma and has a positive clinical response. She has been compliant in taking her medication however,...

09 February 2021 3,489 3 View

Has anybody administered MK-2206 (an orally active, allosteric inhibitor of AKT) intravenously to the mice?

We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...

07 February 2021 6,340 1 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

How to create a graphic with two curve ??

Hello, i want to do a graphic with two curve to compare a toxicity of two metals in spss but i don't know how to do this. Can anyone help

01 February 2021 9,371 7 View

The same lC-MS for Forensic Toxicological and Chemical expertises?

Dear Colleagues! How correct and appropriate is it to carry out a forensic toxicological examination of drugs and psychotropic substances in biological objects (in blood, urine, hair) and a...

24 January 2021 4,489 2 View

GC-Headspace (HS-GC) chromatography for Forensic toxicological and Food expertises?

Dear Colleagues! How correct and appropriate is it to conduct forensic toxicological and food examination of volatile substances (ethanol, methanol, propanol, ethyl glycol, etc.) in blood and...

24 January 2021 5,645 2 View

Which are the appropriate solvents for n-hexane plant extract for in vivo animal models to avoid toxicity?n-hexane plant extract is not soluble?

My n hexane plant extract is not soluble in any solvent. I have tried water, normal saline, tween 20 (2%, 5%, 10%, 50%), tween 80 (2%, 5%, 10%, 50%), pure DMSO, ethanol (2%, 5%, 10%, 50%, 97%),...

16 January 2021 3,547 3 View