I intend to use photocalorimeter to take the OD values of the cell culture tubes. I would like to know if the results would contradict that of UV readings and ultimately the concentration calcultation?
Hi,
I would strongly advise against that approach. Concentrations in suspensions are usually measured at higher wavelengths (> 600 nm), and they only serve as a rough estimation. Also, you would have to trypsinize your adherent cells before the mesurement and make sure they remain in suspension by regular agitation. It is best to stick to proven methods, e.g. using a coulter cell counter. If you dont have one available, a simple hemocytometer and a microscope will do the job, too. Alternatively, use an standard photometer or a microplate reader and set it to an appropriate wavelength (~600 - 700 nm).
Best regards
I agree with the answer above, this approach is highly indirect.
You could use dyes like sulforhodamine B or crystal violet , where you can generate standard curves with increasing cell numbers (counted with a hemocytometer) to establish a linear correlation between absorption and cell numbers. Then you can use those on your experiments.
The above answers provide the easiest way to determine cell densities. A hemocytometer and a microscope.
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