29 December 2024 0 10K Report

Can the Cre-LoxP system be used clinically trial(Field of cell therapy)? Can a residual loxp site be harmful to the human body?Can you share the literature on cell therapy?

More Zhou Qiqi's questions See All
Why the fibroblasts transfected with puromycin plasmids are dying after treatment of puromycin?

Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...

23 June 2024 4,902 3 View

Stable transfection be combined with two or more antibiotics to screen cells?

Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?

10 June 2024 9,739 0 View

What role can community engagement play in heritage preservation and interpretation?

To examine the impact of community engagement in heritage preservation and interpretation

01 June 2024 3,478 11 View

Can sulfate dextran be a substrate for dextranase?

Hello every, I am looking for a commercial enzyme that can degrade sulfate dextran. What is known at present is that dextran can be a substrate for dextranase. so I want to know if sulfate...

23 May 2024 1,471 0 View

[Fairclough’s three dimension (Description+interpretaion) + content analysis ]+ [Fairclough’s (explination) +Willig’s practice +Foucaldian CDA]?

In order to understand whether the two newspapers marginalised one group and diverted attention from other group following a case study. Firstly using first and second levels of Fairclough’s...

23 May 2024 3,208 5 View

Is it easier to couple polypeptides after a few days of dissolving them in PBS?

I want to couple the polypeptide to a cell culture plate, is it easier to couple the polypeptide after a few days of dissolution in PBS?

21 May 2024 9,684 1 View

Do cultured embryonic stem cells or iPSCs migrate spontaneously?

Do embryonic stem cells or iPSCs cultured in vitro on normal culture plates migrate spontaneously without any stimulation?

18 May 2024 6,861 2 View

Why the lateral resolution of OLS5100 (Olympus) can be down to 120 nm?

The laser confocal system of OLS5100 (Olympus) can reach a lateral resolution of 120 nm, that is far beyond the diffraction limitation. Though the numerical aperture of its system is 0.95, the...

15 May 2024 1,458 3 View

How do the Maxent use to prediction the distribution of the cave fishes in Karst area?

As the known, there were the most richness species diversity of the cave animals, especially for the cave fishes, because the underground river sytems. But there were the diffculties exist also,...

14 May 2024 9,131 0 View

Preparation of PC solution?

Hello, everyone, recently I have been transferring two-dimensional materials, using PC transfer, but there is a problem with the configuration of PC solution recently, and I cannot pick up the...

11 May 2024 6,564 0 View

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Approximate concentrations are require in compared with the WHO permissible limts

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

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