Zhou Qiqi

13 Questions 11 Answers 0 Followers

Questions related from Zhou Qiqi

Can the Cre-LoxP system be used clinically trial?Can a residual loxp site be harmful to the human body?Can you share the literature on cell therapy?

Can the Cre-LoxP system be used clinically trial(Field of cell therapy)? Can a residual loxp site be harmful to the human body?Can you share the literature on cell therapy?

29 December 2024 9,911 0 View

Gene editing knock-in experiments, if off-target occurs, can donor still be knocked in?

Off-targeting occurs when the wrong position is cut, so can the donor still be knocked into the wrong position when the homology arm and the sequence at the wrong position are not complementary?

14 November 2024 1,350 0 View

Can the Cre-LoxP system be used clinically trial? Can a residual loxp site be harmful to the human body?

16 October 2024 824 5 View

Can cells be screened for both antibiotics at the same time?

I am doing a gene editing experiment and I want to edit two sites at the same time, so there are two resistance genes, can I add two antibiotics to the cell after transfection 72 hours or do I add...

13 July 2024 7,544 3 View

Stable transfection be combined with two or more antibiotics to screen cells?

Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?

13 June 2024 8,142 0 View

Stable transfection be combined with two or more antibiotics to screen cells?

Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?

11 June 2024 9,819 0 View

Is it easier to couple polypeptides after a few days of dissolving them in PBS?

I want to couple the polypeptide to a cell culture plate, is it easier to couple the polypeptide after a few days of dissolution in PBS?

22 May 2024 9,752 1 View

Do cultured embryonic stem cells or iPSCs migrate spontaneously?

Do embryonic stem cells or iPSCs cultured in vitro on normal culture plates migrate spontaneously without any stimulation?

19 May 2024 6,936 2 View

Is it okay to start with different amounts of RNA in reverse transcription?

The amount of total RNA used by each group was different during reverse transcription. Can it be compensated by adjusting the same CT value during PCR?

30 April 2024 8,766 8 View

Why did someone average the control when performing qpcr calculations?

Why did someone average the control? When selecting your calibrator sample you can select just one of the ‘controls’ that way only 1 sample in the experiment will have a 2^-ddCT of 1.I see a lot...

07 April 2024 3,907 0 View

Do ligands have binding sites?

There are binding sites in the receptor, so do ligands have binding sites? Can you give me an example where the ligand is a protein?

05 March 2024 3,260 4 View

How do receptors and ligands interact?

If I isolate a motif in a domain, is it still functional? Will the amino acid sequence function if I synthesize only the binding site region of the domain?

04 March 2024 981 4 View

If the gene editing（knockin） is off target, can the donor knock in?

If the gene editing（knockin） is off target, can the donor knock in? Aren't homologous arms not complementary? If you can knock in the donor, what's the mechanism?

01 January 1970 8,899 0 View