Zhou Qiqi

7 Questions 8 Answers 0 Followers

Questions related from Zhou Qiqi

Stable transfection be combined with two or more antibiotics to screen cells?

Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?

11 June 2024 9,711 0 View

Is it easier to couple polypeptides after a few days of dissolving them in PBS?

I want to couple the polypeptide to a cell culture plate, is it easier to couple the polypeptide after a few days of dissolution in PBS?

22 May 2024 9,634 1 View

Do cultured embryonic stem cells or iPSCs migrate spontaneously?

Do embryonic stem cells or iPSCs cultured in vitro on normal culture plates migrate spontaneously without any stimulation?

19 May 2024 6,819 2 View

Is it okay to start with different amounts of RNA in reverse transcription?

The amount of total RNA used by each group was different during reverse transcription. Can it be compensated by adjusting the same CT value during PCR?

30 April 2024 8,700 8 View

Why did someone average the control when performing qpcr calculations?

Why did someone average the control? When selecting your calibrator sample you can select just one of the ‘controls’ that way only 1 sample in the experiment will have a 2^-ddCT of 1.I see a lot...

07 April 2024 3,817 0 View

Do ligands have binding sites?

There are binding sites in the receptor, so do ligands have binding sites? Can you give me an example where the ligand is a protein?

05 March 2024 3,183 4 View

How do receptors and ligands interact?

If I isolate a motif in a domain, is it still functional? Will the amino acid sequence function if I synthesize only the binding site region of the domain?

04 March 2024 917 4 View