13 Questions 11 Answers 0 Followers
Questions related from Zhou Qiqi
Can the Cre-LoxP system be used clinically trial(Field of cell therapy)? Can a residual loxp site be harmful to the human body?Can you share the literature on cell therapy?
29 December 2024 9,903 0 View
Off-targeting occurs when the wrong position is cut, so can the donor still be knocked into the wrong position when the homology arm and the sequence at the wrong position are not complementary?
14 November 2024 1,340 0 View
Can the Cre-LoxP system be used clinically trial? Can a residual loxp site be harmful to the human body?
16 October 2024 810 5 View
I am doing a gene editing experiment and I want to edit two sites at the same time, so there are two resistance genes, can I add two antibiotics to the cell after transfection 72 hours or do I add...
13 July 2024 7,533 3 View
Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?
13 June 2024 8,127 0 View
11 June 2024 9,802 0 View
I want to couple the polypeptide to a cell culture plate, is it easier to couple the polypeptide after a few days of dissolution in PBS?
22 May 2024 9,733 1 View
Do embryonic stem cells or iPSCs cultured in vitro on normal culture plates migrate spontaneously without any stimulation?
19 May 2024 6,920 2 View
The amount of total RNA used by each group was different during reverse transcription. Can it be compensated by adjusting the same CT value during PCR?
30 April 2024 8,752 8 View
Why did someone average the control? When selecting your calibrator sample you can select just one of the ‘controls’ that way only 1 sample in the experiment will have a 2^-ddCT of 1.I see a lot...
07 April 2024 3,897 0 View
There are binding sites in the receptor, so do ligands have binding sites? Can you give me an example where the ligand is a protein?
05 March 2024 3,246 4 View
If I isolate a motif in a domain, is it still functional? Will the amino acid sequence function if I synthesize only the binding site region of the domain?
04 March 2024 968 4 View
If the gene editing(knockin) is off target, can the donor knock in? Aren't homologous arms not complementary? If you can knock in the donor, what's the mechanism?
01 January 1970 8,892 0 View