Interpretation of X-ray reflectivity spectrum?

Dear community, I performed XRR measurements on thin film AuAgSi alloys deposited on Si substrate, please see attached image. For simulation i included 2 nm of SiO2 on top of the substrate and i...

03 February 2021 5,325 2 View

What is the best GIS tool to calculate connectivity score for a point along aquatic network?

Hello, I am looking for a GIS tool (ArcGIS, QGIS, R, etc.) that will calculate a measure of habitat connectivity for nodes along an aquatic (estuarine) network? My dataset is comprised of a...

12 January 2021 6,107 2 View

Can I sequence PCR products from green masterm mix without purification?

I wonder if anybody has an experience of sequencing PCR products where master mix contains green dye for gel loading. What if I will purify them with enzymes instead of gel extraction or...

18 May 2020 5,831 5 View

How to calculate circular dichroism in VASP?

Is there an option to calculate circular dichroism in VASP. If not in VASP then is some free open source software. I need to calculate the spectra for small inorganic nanoclusters (100-200 atoms)

07 May 2019 9,377 3 View

Does anyone detect the 'salt effect' when determining ammona in seawater, highly saline water or brine?

Does anyone detect the 'salt effect' when determining ammona in seawater, highly saline water or brine? How does the time of colouration change when using the indophenol blue reaction in salt...

09 January 2019 1,932 5 View

What types of mouse BM cells can adhere?

07 July 2016 9,616 3 View

Why ROC Curves developed by MATLAB tools are not completely consistent with ROC curves plot from "Principles of underwater sound" (Urick, 1983)?

07 July 2016 474 2 View

Can the antibody alone trigger the FcR-mediated effects in macrophages?

In some models of macrophage activation it can be triggered by the binding of immune complexes to Fc receptors. But in reality free antibodies can also bind FcRs (that's why we use the Fc block in...

18 April 2016 5,292 2 View

I'm involved in CVD graphene preparation and I need a gas diagram (Ar, H2 and CH4) for a CVD equipment. Can anyone help me, please?

Actually I have almost everything for the experiment - chemicals, foil, CVD equipment and so on. And here is the thing - how to adjust the flow of gases?Here you can see a part of a receipt from...

16 March 2016 7,373 3 View

Why are my macrophages positive for both CCR7 and CD206?

I have stained mouse BM with F4/80, CD11b, MHCII, CD206 and CCR7. Cells were Fc-blocked. When gated on F4/80_hi/CD11b_med-hi, I see a population that is CCR7+/CD206+ double positive (see...

31 January 2016 1,192 5 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View