Interpretation of X-ray reflectivity spectrum?

Dear community, I performed XRR measurements on thin film AuAgSi alloys deposited on Si substrate, please see attached image. For simulation i included 2 nm of SiO2 on top of the substrate and i...

03 February 2021 5,325 2 View

What is the best GIS tool to calculate connectivity score for a point along aquatic network?

Hello, I am looking for a GIS tool (ArcGIS, QGIS, R, etc.) that will calculate a measure of habitat connectivity for nodes along an aquatic (estuarine) network? My dataset is comprised of a...

12 January 2021 6,107 2 View

Can I sequence PCR products from green masterm mix without purification?

I wonder if anybody has an experience of sequencing PCR products where master mix contains green dye for gel loading. What if I will purify them with enzymes instead of gel extraction or...

18 May 2020 5,831 5 View

How to calculate circular dichroism in VASP?

Is there an option to calculate circular dichroism in VASP. If not in VASP then is some free open source software. I need to calculate the spectra for small inorganic nanoclusters (100-200 atoms)

07 May 2019 9,377 3 View

Does anyone detect the 'salt effect' when determining ammona in seawater, highly saline water or brine?

Does anyone detect the 'salt effect' when determining ammona in seawater, highly saline water or brine? How does the time of colouration change when using the indophenol blue reaction in salt...

09 January 2019 1,932 5 View

What types of mouse BM cells can adhere?

07 July 2016 9,616 3 View

Why ROC Curves developed by MATLAB tools are not completely consistent with ROC curves plot from "Principles of underwater sound" (Urick, 1983)?

07 July 2016 474 2 View

Can the antibody alone trigger the FcR-mediated effects in macrophages?

In some models of macrophage activation it can be triggered by the binding of immune complexes to Fc receptors. But in reality free antibodies can also bind FcRs (that's why we use the Fc block in...

18 April 2016 5,292 2 View

I'm involved in CVD graphene preparation and I need a gas diagram (Ar, H2 and CH4) for a CVD equipment. Can anyone help me, please?

Actually I have almost everything for the experiment - chemicals, foil, CVD equipment and so on. And here is the thing - how to adjust the flow of gases?Here you can see a part of a receipt from...

16 March 2016 7,373 3 View

Why are my macrophages positive for both CCR7 and CD206?

I have stained mouse BM with F4/80, CD11b, MHCII, CD206 and CCR7. Cells were Fc-blocked. When gated on F4/80_hi/CD11b_med-hi, I see a population that is CCR7+/CD206+ double positive (see...

31 January 2016 1,192 5 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Why ICP-MS test for Teflon filters that collected PM2.5, show really high results in the millions of ug/g ?

I analyzed soil and ash samples using ICP-MS for 28 metals, then I resuspended those samples to collect PM2.5 particles in a filter using a selective inlet inlet. My only issue that Fe and Al...

13 February 2021 7,592 3 View

What influences inter-day reproducibility in UPLC-MS/MS analysis?

I was analyzing some metabolites in human plasma. While the intraday precision of these metabolites was acceptable ( less than 20%), the interday precision was quite large. So what influences...

13 February 2021 9,183 9 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

2.6 Drying gas flow controller cannot regulate the requested setting after a fixed amount of time?

We keep getting this error on our Agilent 6470 TQ. We were told it could either be our N2 generator or filter traps. We've called the manufacturer of our generator and they believe it's working...

21 January 2021 2,969 1 View

How to distinguish pure and adulterated honey using NMR spectroscopy?

What are the common adulterants in commercial honey? how to identify pure honey and adulterated honey with the help of NMR spectroscopy? Is there any other technique to check purity of honey?

20 January 2021 5,456 12 View