Oleksandr Galkin

6 Questions 19 Answers 0 Followers

Questions related from Oleksandr Galkin

Alternative methods of depletion for FACS antibody-stained cells?

I need to see the effect of negative depletion of subpopulations expressing different surface markers on my target heterogeneous cell population. There is around 20 markers, for each of them...

16 May 2018 8,141 5 View

Are there any markers of macrophage phagocytic activity?

Are there any known markers whose expression changes significantly upon phagocytosis in macrophages? So you could simply check it's expression and tell which of the cells engulfed something? The...

01 September 2016 6,334 5 View

What types of mouse BM cells can adhere?

If I flush the mouse bone marrow and culture it overnight in DMEM in tissue-culture treated plates, some cells adhere and remain so after the washings. When I Accumax them they come off very...

08 July 2016 9,742 3 View

Can the antibody alone trigger the FcR-mediated effects in macrophages?

In some models of macrophage activation it can be triggered by the binding of immune complexes to Fc receptors. But in reality free antibodies can also bind FcRs (that's why we use the Fc block in...

19 April 2016 5,439 2 View

Why are my macrophages positive for both CCR7 and CD206?

I have stained mouse BM with F4/80, CD11b, MHCII, CD206 and CCR7. Cells were Fc-blocked. When gated on F4/80_hi/CD11b_med-hi, I see a population that is CCR7+/CD206+ double positive (see...

01 February 2016 1,366 5 View

Are there any phagocytosis-associated surface markers of macrophages?

Are there any surface markers of macrophages that are being up/downregulated after macrophages engulf particles via Fc-receptor dependent phagocytosis? Or just upon binding of the antibody to Fc...

29 October 2015 5,844 3 View