what is the best way to extract microbial DNA from this swab type?
I recommend Zymo research extraction kit, you can collect samples and preserve it on PBS
Need sample size calculation formula but no previous studies available, only one study available that have only mean, no standarad deviation available so what formula used in Randomized Controlled...
03 June 2023 9,842 3 View
looking for process/methods/recommendations
13 May 2022 1,493 1 View
What would be the possible elements collected in the Dust Collector after Aluminum Spray by Twin wire ARC and the emission details and likely disposal measures
26 October 2020 8,854 3 View
i tried with the published protocols, but i am not getting any seperation in the ficol gradient. It seems like i am not incubating the 7gm minced tissue for proper time in the digestion...
03 October 2020 8,511 4 View
using carboxyl modified latex edc nhs mediated coupling method is giving very law reaction . is there other latex particles to be used to develop latex reagent or latex modifiaction to couple...
16 January 2020 2,413 0 View
I need to make polyethylene film that allows gas to pass through it under pressure of 10-20 psi, albeit at a very slow rate. Is there in any fillers or some way of formulating the polyethylene so...
17 October 2019 9,037 3 View
About making device of 1 mm crystal.
22 May 2018 1,638 4 View
I have tried to synthesized but not succeed. Will i have to protect OH group? Give me any idea.
10 January 2017 9,122 8 View
There is accumulation of CO2 in the air conditioned spaced over a period of time if the room is not well ventilated. This accumulation of CO2 can be the cause of following: Suffocation,...
26 July 2016 9,693 5 View
I am proposing a study along similar lines, and so was wondering how often would I have to re-new the scents with fresh scent?
21 November 2014 6,237 5 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
We are planning to establish a microbial laboratory in our research center and are actively seeking the best disinfection methods. If anyone has any recommendations, please let us know. Thank you.
04 August 2024 1,973 1 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View