using carboxyl modified latex edc nhs mediated coupling method is giving very law reaction . is there other latex particles to be used to develop latex reagent or latex modifiaction to couple antibody giving highly reactive latex reagent ?
Hi All, I need an opinion about an experiment I am planning to do. I am planning to do a genome wide CRISPR/Cas9 screening to identify genes responsible for drug resistance. I have purchased the...
02 January 2024 6,369 2 View
Need sample size calculation formula but no previous studies available, only one study available that have only mean, no standarad deviation available so what formula used in Randomized Controlled...
03 June 2023 9,842 3 View
looking for process/methods/recommendations
13 May 2022 1,493 1 View
Is there a method to determine the MSI status of mouse cell lines? I saw that most assays (Eg: Bethesda panel) are used for either tumor of human clinical samples. Apart from in depth sequencing...
12 April 2022 1,765 0 View
I've noticed that after treating my suspension cells with a drug that induces apoptosis, the cells become increasingly adherent as the drug concentration increases. Is senescence one of the...
27 October 2020 7,596 1 View
What would be the possible elements collected in the Dust Collector after Aluminum Spray by Twin wire ARC and the emission details and likely disposal measures
26 October 2020 8,854 3 View
i tried with the published protocols, but i am not getting any seperation in the ficol gradient. It seems like i am not incubating the 7gm minced tissue for proper time in the digestion...
03 October 2020 8,511 4 View
what is the best way to extract microbial DNA from this swab type?
29 January 2020 9,102 1 View
I need to make polyethylene film that allows gas to pass through it under pressure of 10-20 psi, albeit at a very slow rate. Is there in any fillers or some way of formulating the polyethylene so...
17 October 2019 9,037 3 View
I am planning to do Co-IP. My lysis buffer has 1mM DTT. If I use lysate with DTT Can this affect my immunoprecipitation experiment ? Can I store immunoprecipitated beads in 4 C for ON till I...
29 April 2019 2,935 4 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
These will be used for the study of microplastics in the lung
29 July 2024 9,609 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hi everyone, I have conducted a mediation analysis with SEM. My model fit was good but there was no significant mediation. Which aspects of this should I discuss?
19 July 2024 4,272 5 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View