Can someone recommend a mouse vasoactive intestinal peptide (VIP) ELISA for cell culture supernatants?

I work in a cell line expressing and storing VIP and look for a robust and sensitive ELISA for VIP in the supernatants (serum-free). Can someone recommend an assay, no matter if commercial or...

05 June 2015 4,456 2 View

Is mouse hippocampal neuronal differentiation in vitro comparable to in vivo?

Many groups use newborn mouse hippocampal cell preparations to analyse differentiation and signaling processes. These cultures are usually kept cultivated for 2 to 4 weeks in vitro before use. Do...

09 October 2014 8,196 6 View

Is it time to have a resource for biological knowledge and understanding like the Beilstein is for Chemistry?

New papers on biological principles and mechanisms appear on a daily basis. May it be necessary or useful to attempt an approach like the Beilstein for Chemicals and Chemistry on a "ten years...

04 May 2014 1,413 2 View

Are there specific or selective activators and/or inhibitors for cyclic nucleotide gated ion channels for use in a cell-culture system?

I observe a clear effect with forskolin and cyclic nucleotide-dependent protein kinase activators (Sp-cAMPS, SpcDDBIMPS), but cannot inhibit that with myr-PKI. The EPAC-specific agonist has no...

06 July 2013 6,473 24 View

Is myr-PKI a good PKA inhibitor in cell culture?

08 September 2012 7,975 13 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View

Can any one suggest the name of Litchi insect/predator?

Is it a type of Ambush bug or what? Thanks

14 January 2021 9,381 3 View