Can someone tell me which insect this is (I am no insect specialist)?

05 June 2016 3,409 6 View

Is mouse hippocampal neuronal differentiation in vitro comparable to in vivo?

Many groups use newborn mouse hippocampal cell preparations to analyse differentiation and signaling processes. These cultures are usually kept cultivated for 2 to 4 weeks in vitro before use. Do...

09 October 2014 8,196 6 View

Is it time to have a resource for biological knowledge and understanding like the Beilstein is for Chemistry?

New papers on biological principles and mechanisms appear on a daily basis. May it be necessary or useful to attempt an approach like the Beilstein for Chemicals and Chemistry on a "ten years...

04 May 2014 1,413 2 View

Are there specific or selective activators and/or inhibitors for cyclic nucleotide gated ion channels for use in a cell-culture system?

I observe a clear effect with forskolin and cyclic nucleotide-dependent protein kinase activators (Sp-cAMPS, SpcDDBIMPS), but cannot inhibit that with myr-PKI. The EPAC-specific agonist has no...

06 July 2013 6,473 24 View

Is myr-PKI a good PKA inhibitor in cell culture?

08 September 2012 7,975 13 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

No title

01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View