I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can someone explain how to interpret correctly?

I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can anybody explain how to interpret it correctly? Thank you in advance

11 January 2021 3,346 2 View

Problem with PCR using plasmid as template?

Hi I am trying to do a PCR for a 1kb fragment using a plasmid of 11kb as a template. But unfortunately on the gel, the band is as large as the plasmid, that is 11kb. Would anyone know that that...

20 October 2020 7,631 3 View

How can I get access to the travaux preparatories of international conventions related to Intellectual Property Rights?

For instance, the 'Historical Archives' section of the 'Audio Visual Library of the International Law' website gives access to the negotiation documents related to various instruments of...

07 June 2020 3,135 3 View

How to do primary cell culture from tumor tissue and injection to mice?

Dear Karen, I am aiming to isolate primary cells from freshly resected tumor tissue from consented patient and injecting those cells to mice. We have failed two times doing so, because the cells...

15 January 2020 8,262 2 View

What are the miRNA profiling alternatives?

I am interested in looking at miRNA signature may be for 10-15miRNAs, but amount of input RNA is limited. qRTPCR is not realistic, since it can provide only one or two miRNA data. Nanostring and...

26 April 2019 9,862 3 View

Rod to spore shaped 'particles' in cell cultures, bacterial contamination?

Last week I had consecutive contaminations of some of my mammalian primary cells alone. It started with the cultures turning turbid after a day or two of incubation. I wiped down the entire...

11 February 2019 5,127 8 View

Is WatchPAT a home sleep monior?

Is WatchPAT from Itamar medical a home sleep monitor or sleep apnea monitor? Can it be used to generate the ground truth for sleep staging? Any reliable references of the use of the device as a...

11 December 2018 1,313 2 View

How to interface arduino to Visual basics 2015?

can someone suggest me any website for visual basics coding or else give tell me how can I learn it quickly

30 November 2018 3,130 1 View

What is the difference between Subject specific and Epoch specific clustering?

Many of the machine learning algorithms use k-means clustering to improve the clasification performance based on human physiological signals. However, while some studies uses a subject specific...

08 November 2018 2,624 1 View

What is the advantage of using statistical techniques before feature selection using wrapper method?

I have seen literature reporting the use of statistical techniques like Mann-Whitney U test and Cohen's D effect size to identify most suitable subset of features for a classifcation problem. But...

15 September 2018 6,687 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View