I am trying to use heart beat as a developmental index to study the effect of environmental contaminants. I will be using MS-222 (Tricaine methanesulfonate) to anesthetize the larvae.
Ms-222 will definitely affect the heart beat. In general to measure it you should take a short video of the heart and measure the image change over time. I recommend this publications.
Article Systolic and diastolic ventricular function in zebrafish emb...
Article Noninvasive technique for measurement of heartbeat regularit...
You can also check
Data Pylatiuk-Automatic Zebrafish Heartbeat Detection and Analysi...
including MATLAB scripts for this task.
hello
maybe these can also help
https://www.nature.com/articles/srep04898
Article Automatic Zebrafish Heartbeat Detection and Analysis for Zeb...
Article Noninvasive technique for measurement of heartbeat regularit...
Good luck
Hi Jacky,
Please check the link below. It would be useful to you.
Article Toxicity assessment of pyriproxyfen in vertebrate model zebr...
Ultrasound (like by the company of Fujifilm - Visualsonics) may be an option to measure the heartbeat. There are devices available for adults, they can probably also used in larvae. But Tricain (MS-222) is affecting the heartbeat, like probably all anesthetics. Depending on size and age of the larvae it may work with just embedding them into soft agarose to immobilize them. Or use a wet sponge with a slit in it to position the larvae.
You can use methyl cellulose for mounting the live zebrafish larvae under microscope. Record the video and data can be analysed using many softwares like danioscope
- Badges
- Science topic
- Similar topics
- Biological Science
- Physiology
- Physiological Processes
- Stress